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Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.

Galaev, Igor LU and Mattiasson, Bo LU (2008) In Methods in Molecular Biology 421. p.247-255
Abstract
Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Affinity: methods, Chromatography
in
Methods in Molecular Biology
volume
421
pages
247 - 255
publisher
Springer
external identifiers
  • pmid:18826059
ISSN
1940-6029
DOI
10.1007/978-1-59745-582-4_16
language
English
LU publication?
yes
id
5a5a9314-9281-4ab4-886f-bb2f9a177162 (old id 1262689)
date added to LUP
2008-11-25 18:20:29
date last changed
2016-04-15 20:35:12
@article{5a5a9314-9281-4ab4-886f-bb2f9a177162,
  abstract     = {Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.},
  author       = {Galaev, Igor and Mattiasson, Bo},
  issn         = {1940-6029},
  keyword      = {Affinity: methods,Chromatography},
  language     = {eng},
  pages        = {247--255},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.},
  url          = {http://dx.doi.org/10.1007/978-1-59745-582-4_16},
  volume       = {421},
  year         = {2008},
}