Advanced

Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes

Johansson, Göte (1996) In Journal of Chromatography. B 680(1-2). p.123-130
Abstract
New aqueous liquid-liquid two-phase systems based on bovine serum albumin and sodium thiocyanate in combination with either poly(vinyl alcohol) or poly(ethylene glycol) were investigated. Phase diagrams are presented. Lactate dehydrogenase and some mitochondrial enzymes were partitioned in the systems. All the phase components used influenced, either positively or negatively, the activity of lactate dehydrogenase. The enzymes showed a strong preference for the albumin phase. Possible scientific and biotechnological uses are discussed.
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous two-phase systems, Partitioning, Proteins, Bovine serum albumin, Polyvinyl alcohol, Poly(ethylene glycol), Lactate dehydrogenase, Enzymes
in
Journal of Chromatography. B
volume
680
issue
1-2
pages
123 - 130
publisher
Elsevier
external identifiers
  • scopus:0029982450
ISSN
1873-376X
DOI
10.1016/0378-4347(95)00449-1
language
English
LU publication?
no
id
72a7816b-e233-41a3-9043-1130a3ac4346 (old id 126287)
date added to LUP
2007-07-05 15:28:49
date last changed
2017-10-22 03:42:04
@article{72a7816b-e233-41a3-9043-1130a3ac4346,
  abstract     = {New aqueous liquid-liquid two-phase systems based on bovine serum albumin and sodium thiocyanate in combination with either poly(vinyl alcohol) or poly(ethylene glycol) were investigated. Phase diagrams are presented. Lactate dehydrogenase and some mitochondrial enzymes were partitioned in the systems. All the phase components used influenced, either positively or negatively, the activity of lactate dehydrogenase. The enzymes showed a strong preference for the albumin phase. Possible scientific and biotechnological uses are discussed.},
  author       = {Johansson, Göte},
  issn         = {1873-376X},
  keyword      = {Aqueous two-phase systems,Partitioning,Proteins,Bovine serum albumin,Polyvinyl alcohol,Poly(ethylene glycol),Lactate dehydrogenase,Enzymes},
  language     = {eng},
  number       = {1-2},
  pages        = {123--130},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes},
  url          = {http://dx.doi.org/10.1016/0378-4347(95)00449-1},
  volume       = {680},
  year         = {1996},
}