Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes
(1996) In Journal of Chromatography. B 680(1-2). p.123-130- Abstract
- New aqueous liquid-liquid two-phase systems based on bovine serum albumin and sodium thiocyanate in combination with either poly(vinyl alcohol) or poly(ethylene glycol) were investigated. Phase diagrams are presented. Lactate dehydrogenase and some mitochondrial enzymes were partitioned in the systems. All the phase components used influenced, either positively or negatively, the activity of lactate dehydrogenase. The enzymes showed a strong preference for the albumin phase. Possible scientific and biotechnological uses are discussed.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126287
- author
- Johansson, Göte
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aqueous two-phase systems, Partitioning, Proteins, Bovine serum albumin, Polyvinyl alcohol, Poly(ethylene glycol), Lactate dehydrogenase, Enzymes
- in
- Journal of Chromatography. B
- volume
- 680
- issue
- 1-2
- pages
- 123 - 130
- publisher
- Elsevier
- external identifiers
-
- scopus:0029982450
- ISSN
- 1572-6495
- DOI
- 10.1016/0378-4347(95)00449-1
- language
- English
- LU publication?
- no
- id
- 72a7816b-e233-41a3-9043-1130a3ac4346 (old id 126287)
- date added to LUP
- 2016-04-01 11:57:17
- date last changed
- 2022-01-26 20:43:52
@article{72a7816b-e233-41a3-9043-1130a3ac4346, abstract = {{New aqueous liquid-liquid two-phase systems based on bovine serum albumin and sodium thiocyanate in combination with either poly(vinyl alcohol) or poly(ethylene glycol) were investigated. Phase diagrams are presented. Lactate dehydrogenase and some mitochondrial enzymes were partitioned in the systems. All the phase components used influenced, either positively or negatively, the activity of lactate dehydrogenase. The enzymes showed a strong preference for the albumin phase. Possible scientific and biotechnological uses are discussed.}}, author = {{Johansson, Göte}}, issn = {{1572-6495}}, keywords = {{Aqueous two-phase systems; Partitioning; Proteins; Bovine serum albumin; Polyvinyl alcohol; Poly(ethylene glycol); Lactate dehydrogenase; Enzymes}}, language = {{eng}}, number = {{1-2}}, pages = {{123--130}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Aqueous two-phase systems with a liquid protein (bovine serum albumin) phase for partitioning of enzymes}}, url = {{http://dx.doi.org/10.1016/0378-4347(95)00449-1}}, doi = {{10.1016/0378-4347(95)00449-1}}, volume = {{680}}, year = {{1996}}, }