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Error associated with experimental flux control coefficient determinations in the Calvin cycle

Pettersson, Gösta LU (1996) In Biochimica et Biophysica Acta. General Subjects 1289(2). p.169-174
Abstract
Model studies of photosynthetic carbohydrate formation in the chloroplast of C3 plants have been performed to examine the expected response of the steady-state reaction flux to finite changes in concentration or activity of individual enzymes in a central metabolic network. The results indicate that flux control coefficients in this system cannot be reliably estimated experimentally even for the two enzymes that do exert predominant control under the examined conditions. Reduction of the mathematical error of the estimates to a satisfactorily low level requires such small enzyme concentration changes that presently available assay methods do not allow for a determination of the corresponding flux changes with satisfactory precision.It is... (More)
Model studies of photosynthetic carbohydrate formation in the chloroplast of C3 plants have been performed to examine the expected response of the steady-state reaction flux to finite changes in concentration or activity of individual enzymes in a central metabolic network. The results indicate that flux control coefficients in this system cannot be reliably estimated experimentally even for the two enzymes that do exert predominant control under the examined conditions. Reduction of the mathematical error of the estimates to a satisfactorily low level requires such small enzyme concentration changes that presently available assay methods do not allow for a determination of the corresponding flux changes with satisfactory precision.It is concluded from these observations and general methodological considerations that experimental flux control coefficient estimates cannot be trusted unless evidence is presented to show that the mathematical error associated with their determination is of insignificant magnitude. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Control analysis, Calvin cycle
in
Biochimica et Biophysica Acta. General Subjects
volume
1289
issue
2
pages
169 - 174
publisher
Elsevier
external identifiers
  • scopus:0029947337
ISSN
0304-4165
DOI
10.1016/0304-4165(95)00078-X
language
English
LU publication?
yes
id
80509d7c-17cf-4427-9e8c-5afd3f7ff875 (old id 126332)
date added to LUP
2007-07-06 14:06:10
date last changed
2017-08-06 04:27:01
@article{80509d7c-17cf-4427-9e8c-5afd3f7ff875,
  abstract     = {Model studies of photosynthetic carbohydrate formation in the chloroplast of C3 plants have been performed to examine the expected response of the steady-state reaction flux to finite changes in concentration or activity of individual enzymes in a central metabolic network. The results indicate that flux control coefficients in this system cannot be reliably estimated experimentally even for the two enzymes that do exert predominant control under the examined conditions. Reduction of the mathematical error of the estimates to a satisfactorily low level requires such small enzyme concentration changes that presently available assay methods do not allow for a determination of the corresponding flux changes with satisfactory precision.It is concluded from these observations and general methodological considerations that experimental flux control coefficient estimates cannot be trusted unless evidence is presented to show that the mathematical error associated with their determination is of insignificant magnitude.},
  author       = {Pettersson, Gösta},
  issn         = {0304-4165},
  keyword      = {Control analysis,Calvin cycle},
  language     = {eng},
  number       = {2},
  pages        = {169--174},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta. General Subjects},
  title        = {Error associated with experimental flux control coefficient determinations in the Calvin cycle},
  url          = {http://dx.doi.org/10.1016/0304-4165(95)00078-X},
  volume       = {1289},
  year         = {1996},
}