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Errors Associated with Experimental Determinations of Enzyme Flux Control Coefficients

Pettersson, Gösta LU (1996) In Journal of Theoretical Biology 179(3). p.191-197
Abstract
An analysis is presented of the likely statistical error and mathematical bias of experimental enzyme flux control coefficient estimates, calculated from observations of the flux changes caused by finite changes of the concentration of an enzyme in a metabolic pathway. The results indicate that such control coefficients are not likely to be determined with satisfactory precision unless the experimental data are evaluated by hyperbolic regression analysis, and the actual flux vs. enzyme concentration profile conforms closely to a hyperbolic relationship. It is concluded that it may not normally be feasible to obtain reliable experimental estimates of enzyme flux control coefficients in realistic systems by the examined direct approach.... (More)
An analysis is presented of the likely statistical error and mathematical bias of experimental enzyme flux control coefficient estimates, calculated from observations of the flux changes caused by finite changes of the concentration of an enzyme in a metabolic pathway. The results indicate that such control coefficients are not likely to be determined with satisfactory precision unless the experimental data are evaluated by hyperbolic regression analysis, and the actual flux vs. enzyme concentration profile conforms closely to a hyperbolic relationship. It is concluded that it may not normally be feasible to obtain reliable experimental estimates of enzyme flux control coefficients in realistic systems by the examined direct approach. Arguments are presented to show that the error situation is unlikely to be more favourable as it concerns that indirect calculation of flux control coefficients from transient-state kinetic data or from experimental estimates of infinitesimally defined quantities such as elasticity coefficients and co-response coefficients. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Theoretical Biology
volume
179
issue
3
pages
191 - 197
publisher
Academic Press
external identifiers
  • scopus:0029885515
ISSN
1095-8541
DOI
10.1006/jtbi.1996.0060
language
English
LU publication?
yes
id
220bae06-701f-47cd-95b8-77c06f087349 (old id 126335)
date added to LUP
2007-07-06 14:04:52
date last changed
2017-01-01 06:47:34
@article{220bae06-701f-47cd-95b8-77c06f087349,
  abstract     = {An analysis is presented of the likely statistical error and mathematical bias of experimental enzyme flux control coefficient estimates, calculated from observations of the flux changes caused by finite changes of the concentration of an enzyme in a metabolic pathway. The results indicate that such control coefficients are not likely to be determined with satisfactory precision unless the experimental data are evaluated by hyperbolic regression analysis, and the actual flux vs. enzyme concentration profile conforms closely to a hyperbolic relationship. It is concluded that it may not normally be feasible to obtain reliable experimental estimates of enzyme flux control coefficients in realistic systems by the examined direct approach. Arguments are presented to show that the error situation is unlikely to be more favourable as it concerns that indirect calculation of flux control coefficients from transient-state kinetic data or from experimental estimates of infinitesimally defined quantities such as elasticity coefficients and co-response coefficients.},
  author       = {Pettersson, Gösta},
  issn         = {1095-8541},
  language     = {eng},
  number       = {3},
  pages        = {191--197},
  publisher    = {Academic Press},
  series       = {Journal of Theoretical Biology},
  title        = {Errors Associated with Experimental Determinations of Enzyme Flux Control Coefficients},
  url          = {http://dx.doi.org/10.1006/jtbi.1996.0060},
  volume       = {179},
  year         = {1996},
}