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Enzyme conformational alterations detected by partition column chromatography

Wingren, Christer LU and Hansson, Ulla-Britt (1996) In Journal of Chromatography. B 680(1-2). p.113-122
Abstract
In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results... (More)
In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Partition column chromatography, Conformational changes, Enzymes
in
Journal of Chromatography. B
volume
680
issue
1-2
pages
113 - 122
publisher
Elsevier
external identifiers
  • scopus:0029894123
ISSN
1572-6495
DOI
10.1016/0378-4347(95)00392-4
language
English
LU publication?
yes
id
ecd7e8a6-b89b-43e4-b4be-f269e6542ad1 (old id 126386)
date added to LUP
2016-04-01 12:25:45
date last changed
2022-01-27 03:38:28
@article{ecd7e8a6-b89b-43e4-b4be-f269e6542ad1,
  abstract     = {{In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.}},
  author       = {{Wingren, Christer and Hansson, Ulla-Britt}},
  issn         = {{1572-6495}},
  keywords     = {{Partition column chromatography; Conformational changes; Enzymes}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{113--122}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Enzyme conformational alterations detected by partition column chromatography}},
  url          = {{http://dx.doi.org/10.1016/0378-4347(95)00392-4}},
  doi          = {{10.1016/0378-4347(95)00392-4}},
  volume       = {{680}},
  year         = {{1996}},
}