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Cell-surface charge and cell-surface hydrophobicity of collagen-binding Aeromonas and Vibrio strains

Ascencio, F; Johansson, G and Wadström, Torkel LU (1995) In Archives of Microbiology 164(3). p.223-230
Abstract
Abstract Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media on the expression of surface properties. There was no apparent relationship between cell-surface charge,... (More)
Abstract Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media on the expression of surface properties. There was no apparent relationship between cell-surface charge, cell-surface hydrophobicity, and binding to collagen. The expression of surface properties was dependent on the culture media. There was no relationship between binding to immobilized collagen and binding to soluble 125I-labeled collagen. Particle-agglutination reactivity differed when using various collagen-coated microbead preparations. There were general differences in the particle-agglutination reactivity when collagen-coated latex beads were prepared using different coating procedures. The negative charge and hydrophobicity of the various collagen-coated microbead preparations were also studied by partitioning in the two-phase system of polyethylene glycol and dextran. Under these conditions, the alpha1+alpha2 collagen-chain mixture covalently immobilized on carboxy-modified latex beads was less hydrophobic and negatively charged than gelatin and native collagen immobilized on the same kind of latex beads. For latex beads passively coated with collagen preparations, the alpha1+alpha2 collagen-chain mixture was more hydrophobic than gelatin and native collagen. We suggest that for screening collagen-binding among Vibrio and Aeromonas strains, a reliable and sensitive particle-agglutination assay should consider the collagen preparation and the coating procedure for the immobilization of collagen onto the latex beads. In this regard, carboxy-modified latex beads coated with an alpha1+alpha2 collagen-chain mixture gave the best results. (Less)
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Contribution to journal
publication status
published
subject
keywords
Key words Cell-surface hydrophobicity . Cell-surface charge . Collagen . Aeromonas caviae . Aeromonas . hydrophila . Aeromonas sobria . Vibrio anguillarum . Vibrio cholerae
in
Archives of Microbiology
volume
164
issue
3
pages
223 - 230
publisher
Springer
external identifiers
  • scopus:0029122932
ISSN
0302-8933
DOI
10.1007/s002030050258
language
English
LU publication?
yes
id
63f53e5e-e107-4108-8d18-47764236c4a4 (old id 126397)
date added to LUP
2007-07-04 10:30:47
date last changed
2017-07-23 04:41:23
@article{63f53e5e-e107-4108-8d18-47764236c4a4,
  abstract     = {Abstract Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media on the expression of surface properties. There was no apparent relationship between cell-surface charge, cell-surface hydrophobicity, and binding to collagen. The expression of surface properties was dependent on the culture media. There was no relationship between binding to immobilized collagen and binding to soluble 125I-labeled collagen. Particle-agglutination reactivity differed when using various collagen-coated microbead preparations. There were general differences in the particle-agglutination reactivity when collagen-coated latex beads were prepared using different coating procedures. The negative charge and hydrophobicity of the various collagen-coated microbead preparations were also studied by partitioning in the two-phase system of polyethylene glycol and dextran. Under these conditions, the alpha1+alpha2 collagen-chain mixture covalently immobilized on carboxy-modified latex beads was less hydrophobic and negatively charged than gelatin and native collagen immobilized on the same kind of latex beads. For latex beads passively coated with collagen preparations, the alpha1+alpha2 collagen-chain mixture was more hydrophobic than gelatin and native collagen. We suggest that for screening collagen-binding among Vibrio and Aeromonas strains, a reliable and sensitive particle-agglutination assay should consider the collagen preparation and the coating procedure for the immobilization of collagen onto the latex beads. In this regard, carboxy-modified latex beads coated with an alpha1+alpha2 collagen-chain mixture gave the best results.},
  author       = {Ascencio, F and Johansson, G and Wadström, Torkel},
  issn         = {0302-8933},
  keyword      = {Key words Cell-surface hydrophobicity . Cell-surface charge . Collagen . Aeromonas caviae . Aeromonas . hydrophila . Aeromonas sobria . Vibrio anguillarum . Vibrio cholerae},
  language     = {eng},
  number       = {3},
  pages        = {223--230},
  publisher    = {Springer},
  series       = {Archives of Microbiology},
  title        = {Cell-surface charge and cell-surface hydrophobicity of collagen-binding Aeromonas and Vibrio strains},
  url          = {http://dx.doi.org/10.1007/s002030050258},
  volume       = {164},
  year         = {1995},
}