Advanced

Characterization of eight barley xantha-f mutants deficient in magnesium chelatase

Olsson, Ulf LU ; Sirijovski, Nick LU and Hansson, Mats LU (2004) In Plant Physiology and Biochemistry 42(6). p.557-564
Abstract
Magnesium chelatase (EC 6.6.1.1) catalyses the insertion of magnesium into protoporphyrin IX, the first unique step of the chlorophyll biosynthetic pathway. The enzyme is composed of three different subunits of approximately 40, 70 and 140 kDa. In barley (Hordeum vulgare L.) the subunits are encoded by the genes Xantha-h, Xantha-g and Xantha-f. In the 1950s, eight induced xantha-f mutants were isolated. In this work we characterized these mutations at the DNA level and provided explanations for their phenotypes. The xantha-f10 mutation is a 3 bp deletion, resulting in a polypeptide lacking the glutamate residue at position 424. The leaky mutation xantha-f26 has a missense mutation leading to a M632R exchange. The xantha-f27 and -f40 are... (More)
Magnesium chelatase (EC 6.6.1.1) catalyses the insertion of magnesium into protoporphyrin IX, the first unique step of the chlorophyll biosynthetic pathway. The enzyme is composed of three different subunits of approximately 40, 70 and 140 kDa. In barley (Hordeum vulgare L.) the subunits are encoded by the genes Xantha-h, Xantha-g and Xantha-f. In the 1950s, eight induced xantha-f mutants were isolated. In this work we characterized these mutations at the DNA level and provided explanations for their phenotypes. The xantha-f10 mutation is a 3 bp deletion, resulting in a polypeptide lacking the glutamate residue at position 424. The leaky mutation xantha-f26 has a missense mutation leading to a M632R exchange. The xantha-f27 and -f40 are deletions of 14 and 2 bp, respectively, resulting in truncated polypeptides of 1104 and 899 amino acid residues, respectively. Mutation xantha-f41 is an in-frame deletion that removes A439, L440, Q441 and V442 from the resulting protein. Mutation xantha-f58 is most likely a deletion of the whole Xantha-f gene, as no DNA fragments could be detected by PCR or southern blot experiments. The slightly leaky xantha-f60 and non-leaky -f68 mutations each have a missense mutation causing a P393L and G794E exchange in the polypeptide, respectively. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bchH, chlH, Mutation, Mg-chelatase, Hordeum vulgare, Protoporphyrin IX
in
Plant Physiology and Biochemistry
volume
42
issue
6
pages
557 - 564
publisher
Elsevier
external identifiers
  • pmid:15246070
  • wos:000223006000013
  • scopus:2942615635
ISSN
1873-2690
DOI
10.1016/j.plaphy.2004.05.011
language
English
LU publication?
yes
id
1db3a496-a159-4ca1-b7b1-41e21f1d031e (old id 126471)
date added to LUP
2007-07-09 08:58:56
date last changed
2017-09-24 04:31:48
@article{1db3a496-a159-4ca1-b7b1-41e21f1d031e,
  abstract     = {Magnesium chelatase (EC 6.6.1.1) catalyses the insertion of magnesium into protoporphyrin IX, the first unique step of the chlorophyll biosynthetic pathway. The enzyme is composed of three different subunits of approximately 40, 70 and 140 kDa. In barley (Hordeum vulgare L.) the subunits are encoded by the genes Xantha-h, Xantha-g and Xantha-f. In the 1950s, eight induced xantha-f mutants were isolated. In this work we characterized these mutations at the DNA level and provided explanations for their phenotypes. The xantha-f10 mutation is a 3 bp deletion, resulting in a polypeptide lacking the glutamate residue at position 424. The leaky mutation xantha-f26 has a missense mutation leading to a M632R exchange. The xantha-f27 and -f40 are deletions of 14 and 2 bp, respectively, resulting in truncated polypeptides of 1104 and 899 amino acid residues, respectively. Mutation xantha-f41 is an in-frame deletion that removes A439, L440, Q441 and V442 from the resulting protein. Mutation xantha-f58 is most likely a deletion of the whole Xantha-f gene, as no DNA fragments could be detected by PCR or southern blot experiments. The slightly leaky xantha-f60 and non-leaky -f68 mutations each have a missense mutation causing a P393L and G794E exchange in the polypeptide, respectively.},
  author       = {Olsson, Ulf and Sirijovski, Nick and Hansson, Mats},
  issn         = {1873-2690},
  keyword      = {bchH,chlH,Mutation,Mg-chelatase,Hordeum vulgare,Protoporphyrin IX},
  language     = {eng},
  number       = {6},
  pages        = {557--564},
  publisher    = {Elsevier},
  series       = {Plant Physiology and Biochemistry},
  title        = {Characterization of eight barley xantha-f mutants deficient in magnesium chelatase},
  url          = {http://dx.doi.org/10.1016/j.plaphy.2004.05.011},
  volume       = {42},
  year         = {2004},
}