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Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases

Jönsson, Leif; Sjöström, Kjell; Häggström, Ingrid and Nyman, Per-Olof LU (1995) In BBA - Protein Structure and Molecular Enzymology 1251(2). p.210-215
Abstract
A gene coding for the multi-copper phenol oxidase laccase has been isolated from the white-rot basidiomycete Trametes oersicolor. The gene, which is preceded by a TATA box and a pyrimidine-rich region, is predicted to contain ten introns. The mature translation product, preceded by a 22-residue signal peptide, should consist of 498 residues. Comparisons with Edman degradation data of peptides from T. versicolor laccase strongly suggest that two disulfide bridges are formed by Cys-85/Cys-487 and Cys-117/Cys-205, respectively. The encoded protein contains five Cys, and the sequence surrounding the remaining Cys-452 is consistent with its involvement in the ligation of type-1 copper. Alignment of sequences indicates that T. versicolor laccase... (More)
A gene coding for the multi-copper phenol oxidase laccase has been isolated from the white-rot basidiomycete Trametes oersicolor. The gene, which is preceded by a TATA box and a pyrimidine-rich region, is predicted to contain ten introns. The mature translation product, preceded by a 22-residue signal peptide, should consist of 498 residues. Comparisons with Edman degradation data of peptides from T. versicolor laccase strongly suggest that two disulfide bridges are formed by Cys-85/Cys-487 and Cys-117/Cys-205, respectively. The encoded protein contains five Cys, and the sequence surrounding the remaining Cys-452 is consistent with its involvement in the ligation of type-1 copper. Alignment of sequences indicates that T. versicolor laccase displays a Phe at the position corresponding to a residue (Met in ascorbate oxidase and azurin) considered important for the reduction potential of type-1 copper proteins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Laccase, Phenol oxidase, Multi-copper enzyme, White-rot fungus, Lignin degradation, (T. versicolor)
in
BBA - Protein Structure and Molecular Enzymology
volume
1251
issue
2
pages
210 - 215
publisher
Elsevier
external identifiers
  • scopus:0029086873
ISSN
0167-4838
DOI
10.1016/0167-4838(95)00104-3
language
English
LU publication?
yes
id
056c3027-6df8-4866-be18-e18189f306a5 (old id 126496)
date added to LUP
2007-07-05 16:18:12
date last changed
2017-08-27 05:40:10
@article{056c3027-6df8-4866-be18-e18189f306a5,
  abstract     = {A gene coding for the multi-copper phenol oxidase laccase has been isolated from the white-rot basidiomycete Trametes oersicolor. The gene, which is preceded by a TATA box and a pyrimidine-rich region, is predicted to contain ten introns. The mature translation product, preceded by a 22-residue signal peptide, should consist of 498 residues. Comparisons with Edman degradation data of peptides from T. versicolor laccase strongly suggest that two disulfide bridges are formed by Cys-85/Cys-487 and Cys-117/Cys-205, respectively. The encoded protein contains five Cys, and the sequence surrounding the remaining Cys-452 is consistent with its involvement in the ligation of type-1 copper. Alignment of sequences indicates that T. versicolor laccase displays a Phe at the position corresponding to a residue (Met in ascorbate oxidase and azurin) considered important for the reduction potential of type-1 copper proteins.},
  author       = {Jönsson, Leif and Sjöström, Kjell and Häggström, Ingrid and Nyman, Per-Olof},
  issn         = {0167-4838},
  keyword      = {Laccase,Phenol oxidase,Multi-copper enzyme,White-rot fungus,Lignin degradation,(T. versicolor)},
  language     = {eng},
  number       = {2},
  pages        = {210--215},
  publisher    = {Elsevier},
  series       = {BBA - Protein Structure and Molecular Enzymology},
  title        = {Characterization of a laccase gene from the white-rot fungus Trametes versicolor and structural features of basidiomycete laccases},
  url          = {http://dx.doi.org/10.1016/0167-4838(95)00104-3},
  volume       = {1251},
  year         = {1995},
}