A new approach to examine conformational changes occurring upon binding of ligand by biomolecules
(1995) In Biochimica et Biophysica Acta. General Subjects 1244(1). p.209-215- Abstract
- Liquid-liquid partition chromatography in an aqueous poly(ethylene glycol)/dextran two-phase system (LLPC) is shown to be a quick and sensitive method for detecting conformational changes occurring upon binding of ligands by biospecific molecules. Two groups of well-characterized proteins, enzymes and monoclonal antibodies, were employed. As an example, LLPC demonstrated that isoforms of lactate dehydrogenase as well as of hexokinase existed in a ligand-dependent equilibrium between two forms and that conformational changes occurred when monoclonal antibodies bound haptens. We also demonstrate that the method could be used to detect and separate subfractions in preparations of unliganded proteins that appeared to be homogeneous when... (More)
- Liquid-liquid partition chromatography in an aqueous poly(ethylene glycol)/dextran two-phase system (LLPC) is shown to be a quick and sensitive method for detecting conformational changes occurring upon binding of ligands by biospecific molecules. Two groups of well-characterized proteins, enzymes and monoclonal antibodies, were employed. As an example, LLPC demonstrated that isoforms of lactate dehydrogenase as well as of hexokinase existed in a ligand-dependent equilibrium between two forms and that conformational changes occurred when monoclonal antibodies bound haptens. We also demonstrate that the method could be used to detect and separate subfractions in preparations of unliganded proteins that appeared to be homogeneous when analysed by other techniques. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126508
- author
- Wingren, Christer LU and Hansson, Ulla-Britt
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Conformational change, Phase partition, LLPC, Ligand binding, Antibody, Enzyme
- in
- Biochimica et Biophysica Acta. General Subjects
- volume
- 1244
- issue
- 1
- pages
- 209 - 215
- publisher
- Elsevier
- external identifiers
-
- scopus:0029011518
- ISSN
- 0304-4165
- DOI
- 10.1016/0304-4165(95)00007-X
- language
- English
- LU publication?
- yes
- id
- f5041780-d8f9-4f58-8e8c-e26b447eb643 (old id 126508)
- date added to LUP
- 2016-04-01 16:12:25
- date last changed
- 2021-01-03 10:09:11
@article{f5041780-d8f9-4f58-8e8c-e26b447eb643, abstract = {{Liquid-liquid partition chromatography in an aqueous poly(ethylene glycol)/dextran two-phase system (LLPC) is shown to be a quick and sensitive method for detecting conformational changes occurring upon binding of ligands by biospecific molecules. Two groups of well-characterized proteins, enzymes and monoclonal antibodies, were employed. As an example, LLPC demonstrated that isoforms of lactate dehydrogenase as well as of hexokinase existed in a ligand-dependent equilibrium between two forms and that conformational changes occurred when monoclonal antibodies bound haptens. We also demonstrate that the method could be used to detect and separate subfractions in preparations of unliganded proteins that appeared to be homogeneous when analysed by other techniques.}}, author = {{Wingren, Christer and Hansson, Ulla-Britt}}, issn = {{0304-4165}}, keywords = {{Conformational change; Phase partition; LLPC; Ligand binding; Antibody; Enzyme}}, language = {{eng}}, number = {{1}}, pages = {{209--215}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta. General Subjects}}, title = {{A new approach to examine conformational changes occurring upon binding of ligand by biomolecules}}, url = {{http://dx.doi.org/10.1016/0304-4165(95)00007-X}}, doi = {{10.1016/0304-4165(95)00007-X}}, volume = {{1244}}, year = {{1995}}, }