Advanced

Quantification of polyphosphoinositides using selected ion monitoring electrospray mass spectrometry

Michelsen, P; Jergil, Bengt LU and Odham, G (1995) In Rapid Communications in Mass Spectrometry 9(12). p.1109-1114
Abstract
Polyphosphoinositides (PIP) and (PIP2) show prominent negative singly and doubly charged deprotonated molecules in electrospray mass spectrometry. These ions can be used for quantification of PIP and PIP2 in the low picomole range, without prior chromatographic separation, using selected ion monitoring and consecutive measurements of the signals from the deprotonated singly charged molecules. The dose response curves for both compounds are linear. In a complex matrix consisting of polar lipids (Folch extract) PIP and PIP2 monitored at m/z 965.4 and 1045.5 (stearoyl and arachidonoyl) were determined in the low picomole range, at a flow rate of 100 mu L/min, Collision-induced decomposition of PIP and PIP2 using a mixture of xenon and argon... (More)
Polyphosphoinositides (PIP) and (PIP2) show prominent negative singly and doubly charged deprotonated molecules in electrospray mass spectrometry. These ions can be used for quantification of PIP and PIP2 in the low picomole range, without prior chromatographic separation, using selected ion monitoring and consecutive measurements of the signals from the deprotonated singly charged molecules. The dose response curves for both compounds are linear. In a complex matrix consisting of polar lipids (Folch extract) PIP and PIP2 monitored at m/z 965.4 and 1045.5 (stearoyl and arachidonoyl) were determined in the low picomole range, at a flow rate of 100 mu L/min, Collision-induced decomposition of PIP and PIP2 using a mixture of xenon and argon at 25 eV afforded identical high mass ions formed by loss of a molecule of water from PIP and a phosphate group and a molecule of water from PIP2. The results indicate that polyphosphoinositides, and biologically relevant changes in their concentrations, can be quantified directly in cells and cellular membranes by selected-ion monitoring with electrospray mass spectrometry. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PHOSPHOINOSITIDE KINASES, DIACYLGLYCEROL KINASE, CELLS, CHROMATOGRAPHY, PHOSPHOLIPASE-C
in
Rapid Communications in Mass Spectrometry
volume
9
issue
12
pages
1109 - 1114
publisher
John Wiley & Sons
external identifiers
  • scopus:85011045864
ISSN
1097-0231
DOI
10.1002/rcm.1290091206
language
English
LU publication?
yes
id
dbd1b82a-cb9b-42d6-9cbd-d76c8cb3eec9 (old id 126517)
date added to LUP
2007-07-06 10:17:43
date last changed
2017-10-01 03:46:56
@article{dbd1b82a-cb9b-42d6-9cbd-d76c8cb3eec9,
  abstract     = {Polyphosphoinositides (PIP) and (PIP2) show prominent negative singly and doubly charged deprotonated molecules in electrospray mass spectrometry. These ions can be used for quantification of PIP and PIP2 in the low picomole range, without prior chromatographic separation, using selected ion monitoring and consecutive measurements of the signals from the deprotonated singly charged molecules. The dose response curves for both compounds are linear. In a complex matrix consisting of polar lipids (Folch extract) PIP and PIP2 monitored at m/z 965.4 and 1045.5 (stearoyl and arachidonoyl) were determined in the low picomole range, at a flow rate of 100 mu L/min, Collision-induced decomposition of PIP and PIP2 using a mixture of xenon and argon at 25 eV afforded identical high mass ions formed by loss of a molecule of water from PIP and a phosphate group and a molecule of water from PIP2. The results indicate that polyphosphoinositides, and biologically relevant changes in their concentrations, can be quantified directly in cells and cellular membranes by selected-ion monitoring with electrospray mass spectrometry.},
  author       = {Michelsen, P and Jergil, Bengt and Odham, G},
  issn         = {1097-0231},
  keyword      = {PHOSPHOINOSITIDE KINASES,DIACYLGLYCEROL KINASE,CELLS,CHROMATOGRAPHY,PHOSPHOLIPASE-C},
  language     = {eng},
  number       = {12},
  pages        = {1109--1114},
  publisher    = {John Wiley & Sons},
  series       = {Rapid Communications in Mass Spectrometry},
  title        = {Quantification of polyphosphoinositides using selected ion monitoring electrospray mass spectrometry},
  url          = {http://dx.doi.org/10.1002/rcm.1290091206},
  volume       = {9},
  year         = {1995},
}