Advanced

Presence of a novel form of phosphatidylinositol 4-kinase in rat liver

Olsson, Henric; Martinez Arias, Wilma LU ; Drøbak, Bjørn K and Jergil, Bengt LU (1995) In FEBS Letters 361(2-3). p.282-286
Abstract
Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of... (More)
Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Signal transduction, Phosphoinositide, Phosphatidyl inositol 4-kinase, Rat liver
in
FEBS Letters
volume
361
issue
2-3
pages
282 - 286
publisher
Wiley-Blackwell
external identifiers
  • scopus:0028933680
ISSN
1873-3468
DOI
10.1016/0014-5793(95)00194-E
language
English
LU publication?
yes
id
ba34c6d4-c8dd-4fc4-a717-3cafc6ab7e02 (old id 126521)
date added to LUP
2007-07-09 09:03:54
date last changed
2017-01-01 07:18:09
@article{ba34c6d4-c8dd-4fc4-a717-3cafc6ab7e02,
  abstract     = {Rat liver microsomes contain two distinct forms of PtdIns 4-kinase which were resolved by heparin-Sepharose chromatography. One enzyme was identified as the type II PtdIns kinase previously isolated from exocytotic vesicles. The other enzyme, however, was a novel PtdIns 4-kinase isoform with properties differing from any other PtdIns kinase so far characterized. Both kinases were recognized by a monoclonal antibody specific for type II PtdIns 4-kinase, but the novel enzyme was considerably less sensitive to inhibition by adenosine and Ca2+ than type II enzymes, and in addition was specifically inhibited by submillimolar concentrations of dithioerythritol. The presence of a novel PtdIns 4-kinase isoform in rat liver raises the question of whether this enzyme is unique for this organ or whether it has a more widespread distribution but so far has avoided detection.},
  author       = {Olsson, Henric and Martinez Arias, Wilma and Drøbak, Bjørn K and Jergil, Bengt},
  issn         = {1873-3468},
  keyword      = {Signal transduction,Phosphoinositide,Phosphatidyl inositol 4-kinase,Rat liver},
  language     = {eng},
  number       = {2-3},
  pages        = {282--286},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Presence of a novel form of phosphatidylinositol 4-kinase in rat liver},
  url          = {http://dx.doi.org/10.1016/0014-5793(95)00194-E},
  volume       = {361},
  year         = {1995},
}