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The purification of membranes by affinity partitioning

Persson, A and Jergil, Bengt LU (1995) In FASEB Journal 9(13). p.1304-1310
Abstract
We describe affinity partitioning as a preparative method for membranes and membraneous structures such as organelles, cells, and viruses. Biospecific affinity partitioning is carried out in aqueous polymer two- phase systems, commonly with polyethylene glycol and dextran as phase polymers, in an environment compatible with membrane structures. Ideally, two-phase conditions are chosen to partition the bulk of membrane material into one phase, while the affinity ligand, conjugated to the second phase polymer, will selectively pull the membranes to be isolated into this phase. Suitable ligands include lectins, antibodies, and receptor-specific agents. Because the method has so far been used successfully in rather few instances, all using... (More)
We describe affinity partitioning as a preparative method for membranes and membraneous structures such as organelles, cells, and viruses. Biospecific affinity partitioning is carried out in aqueous polymer two- phase systems, commonly with polyethylene glycol and dextran as phase polymers, in an environment compatible with membrane structures. Ideally, two-phase conditions are chosen to partition the bulk of membrane material into one phase, while the affinity ligand, conjugated to the second phase polymer, will selectively pull the membranes to be isolated into this phase. Suitable ligands include lectins, antibodies, and receptor-specific agents. Because the method has so far been used successfully in rather few instances, all using high ligand receptor densities in target membranes, the discussion focuses on factors to be considered when developing affinity partitioning conditions using new ligands. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FASEB Journal
volume
9
issue
13
pages
1304 - 1310
publisher
The Federation of American Societies for Experimental Biology
external identifiers
  • scopus:0028892420
ISSN
1530-6860
language
English
LU publication?
yes
id
0965a9cf-b83a-4b2d-a38e-cbce3a23d967 (old id 126527)
alternative location
http://www.fasebj.org/cgi/reprint/9/13/1304
date added to LUP
2007-07-06 12:45:39
date last changed
2017-10-08 04:10:14
@article{0965a9cf-b83a-4b2d-a38e-cbce3a23d967,
  abstract     = {We describe affinity partitioning as a preparative method for membranes and membraneous structures such as organelles, cells, and viruses. Biospecific affinity partitioning is carried out in aqueous polymer two- phase systems, commonly with polyethylene glycol and dextran as phase polymers, in an environment compatible with membrane structures. Ideally, two-phase conditions are chosen to partition the bulk of membrane material into one phase, while the affinity ligand, conjugated to the second phase polymer, will selectively pull the membranes to be isolated into this phase. Suitable ligands include lectins, antibodies, and receptor-specific agents. Because the method has so far been used successfully in rather few instances, all using high ligand receptor densities in target membranes, the discussion focuses on factors to be considered when developing affinity partitioning conditions using new ligands.},
  author       = {Persson, A and Jergil, Bengt},
  issn         = {1530-6860},
  language     = {eng},
  number       = {13},
  pages        = {1304--1310},
  publisher    = {The Federation of American Societies for Experimental Biology},
  series       = {FASEB Journal},
  title        = {The purification of membranes by affinity partitioning},
  volume       = {9},
  year         = {1995},
}