Advanced

Diffusion Dynamics of Motor-Driven Transport: Gradient Production and Self-Organization of Surfaces.

Vikhorev, Petr; Vikhoreva, Natalia; Sundberg, Mark; Balaz, Martina; Albet-Torres, Nuria; Bunk, Richard; Kvennefors, Anders LU ; Liljesson, Kenneth; Nicholls, Ian and Nilsson, Leif, et al. (2008) In Langmuir 24(23). p.13509-13517
Abstract
The interaction between cytoskeletal filaments (e.g., actin filaments) and molecular motors (e.g., myosin) is the basis for many aspects of cell motility and organization of the cell interior. In the in vitro motility assay (IVMA), cytoskeletal filaments are observed while being propelled by molecular motors adsorbed to artificial surfaces (e.g., in studies of motor function). Here we integrate ideas that cytoskeletal filaments may be used as nanoscale templates in nanopatterning with a novel approach for the production of surface gradients of biomolecules and nanoscale topographical features. The production of such gradients is challenging but of increasing interest (e.g., in cell biology). First, we show that myosin-induced actin... (More)
The interaction between cytoskeletal filaments (e.g., actin filaments) and molecular motors (e.g., myosin) is the basis for many aspects of cell motility and organization of the cell interior. In the in vitro motility assay (IVMA), cytoskeletal filaments are observed while being propelled by molecular motors adsorbed to artificial surfaces (e.g., in studies of motor function). Here we integrate ideas that cytoskeletal filaments may be used as nanoscale templates in nanopatterning with a novel approach for the production of surface gradients of biomolecules and nanoscale topographical features. The production of such gradients is challenging but of increasing interest (e.g., in cell biology). First, we show that myosin-induced actin filament sliding in the IVMA can be approximately described as persistent random motion with a diffusion coefficient ( D) given by a relationship analogous to the Einstein equation ( D = kT/gamma). In this relationship, the thermal energy ( kT) and the drag coefficient (gamma) are substituted by a parameter related to the free-energy transduction by actomyosin and the actomyosin dissociation rate constant, respectively. We then demonstrate how the persistent random motion of actin filaments can be exploited in conceptually novel methods for the production of actin filament density gradients of predictable shapes. Because of regularly spaced binding sites (e.g., lysines and cysteines) the actin filaments act as suitable nanoscale scaffolds for other biomolecules (tested for fibronectin) or nanoparticles. This forms the basis for secondary chemical and topographical gradients with implications for cell biological studies and biosensing. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
24
issue
23
pages
13509 - 13517
publisher
The American Chemical Society
external identifiers
  • wos:000261216500043
  • pmid:18989944
  • scopus:62649141432
ISSN
0743-7463
DOI
10.1021/la8016112
language
English
LU publication?
yes
id
7d8cecb3-e3aa-42f5-bf3b-a4215f44a186 (old id 1271753)
date added to LUP
2009-02-10 09:47:43
date last changed
2017-10-01 03:48:33
@article{7d8cecb3-e3aa-42f5-bf3b-a4215f44a186,
  abstract     = {The interaction between cytoskeletal filaments (e.g., actin filaments) and molecular motors (e.g., myosin) is the basis for many aspects of cell motility and organization of the cell interior. In the in vitro motility assay (IVMA), cytoskeletal filaments are observed while being propelled by molecular motors adsorbed to artificial surfaces (e.g., in studies of motor function). Here we integrate ideas that cytoskeletal filaments may be used as nanoscale templates in nanopatterning with a novel approach for the production of surface gradients of biomolecules and nanoscale topographical features. The production of such gradients is challenging but of increasing interest (e.g., in cell biology). First, we show that myosin-induced actin filament sliding in the IVMA can be approximately described as persistent random motion with a diffusion coefficient ( D) given by a relationship analogous to the Einstein equation ( D = kT/gamma). In this relationship, the thermal energy ( kT) and the drag coefficient (gamma) are substituted by a parameter related to the free-energy transduction by actomyosin and the actomyosin dissociation rate constant, respectively. We then demonstrate how the persistent random motion of actin filaments can be exploited in conceptually novel methods for the production of actin filament density gradients of predictable shapes. Because of regularly spaced binding sites (e.g., lysines and cysteines) the actin filaments act as suitable nanoscale scaffolds for other biomolecules (tested for fibronectin) or nanoparticles. This forms the basis for secondary chemical and topographical gradients with implications for cell biological studies and biosensing.},
  author       = {Vikhorev, Petr and Vikhoreva, Natalia and Sundberg, Mark and Balaz, Martina and Albet-Torres, Nuria and Bunk, Richard and Kvennefors, Anders and Liljesson, Kenneth and Nicholls, Ian and Nilsson, Leif and Omling, Pär and Tågerud, Sven and Montelius, Lars and Månsson, Alf},
  issn         = {0743-7463},
  language     = {eng},
  number       = {23},
  pages        = {13509--13517},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Diffusion Dynamics of Motor-Driven Transport: Gradient Production and Self-Organization of Surfaces.},
  url          = {http://dx.doi.org/10.1021/la8016112},
  volume       = {24},
  year         = {2008},
}