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An analysis of population genetic differentiation and genotype-phenotype association across the hybrid zone of carrion and hooded crows using microsatellites and MC1R.

Haas, Fredrik LU ; Pointer, Marie A; Saino, Nicola; Brodin, Anders LU ; Mundy, Nicholas I and Hansson, Bengt LU (2009) In Molecular Ecology 18(2). p.294-305
Abstract
Abstract The all black carrion crow (Corvus corone corone) and the grey and black hooded crow (Corvus corone cornix) meet in a narrow hybrid zone across Europe. To evaluate the degree of genetic differentiation over the hybrid zone, we genotyped crows from the centre and edges of the zone, and from allopatric populations in northern (Scotland-Denmark-Sweden) and southern Europe (western-central northern Italy), at 18 microsatellites and at a plumage candidate gene, the MC1R gene. Allopatric and edge populations were significantly differentiated on microsatellites, and populations were isolated by distance over the hybrid zone in Italy. Single-locus analyses showed that one locus, CmeH9, differentiated populations on different sides of the... (More)
Abstract The all black carrion crow (Corvus corone corone) and the grey and black hooded crow (Corvus corone cornix) meet in a narrow hybrid zone across Europe. To evaluate the degree of genetic differentiation over the hybrid zone, we genotyped crows from the centre and edges of the zone, and from allopatric populations in northern (Scotland-Denmark-Sweden) and southern Europe (western-central northern Italy), at 18 microsatellites and at a plumage candidate gene, the MC1R gene. Allopatric and edge populations were significantly differentiated on microsatellites, and populations were isolated by distance over the hybrid zone in Italy. Single-locus analyses showed that one locus, CmeH9, differentiated populations on different sides of the zone at the same time as showing only weak separation of populations on the same side of the zone. Within the hybrid zone there was no differentiation of phenotypes at CmeH9 or at the set of microsatellites, no excess of heterozygotes among hybrids and low levels of linkage disequilibrium between markers. We did not detect any association between phenotypes and nucleotide variation at MC1R, and the two most common haplotypes occurred in very similar frequencies in carrion and hooded crows. That we found a similar degree of genetic differentiation between allopatric and edge populations irrespectively of their location in relation to the hybrid zone, no differentiation between phenotypes within the hybrid zone, and neither heterozygote excess nor consistent linkage disequilibrium in the hybrid zone, is striking considering that carrion and hooded crows are phenotypically distinct and sometimes recognised as separate species. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
population structure, Corvus corone, hybridisation, Corvidae, introgression
in
Molecular Ecology
volume
18
issue
2
pages
294 - 305
publisher
Wiley-Blackwell
external identifiers
  • wos:000262478700010
  • scopus:58249117731
ISSN
0962-1083
DOI
10.1111/j.1365-294X.2008.04017.x
language
English
LU publication?
yes
id
94d04b6c-da34-46b8-8646-dad2dc5803ea (old id 1276226)
date added to LUP
2009-01-19 13:39:27
date last changed
2017-02-26 03:31:18
@article{94d04b6c-da34-46b8-8646-dad2dc5803ea,
  abstract     = {Abstract The all black carrion crow (Corvus corone corone) and the grey and black hooded crow (Corvus corone cornix) meet in a narrow hybrid zone across Europe. To evaluate the degree of genetic differentiation over the hybrid zone, we genotyped crows from the centre and edges of the zone, and from allopatric populations in northern (Scotland-Denmark-Sweden) and southern Europe (western-central northern Italy), at 18 microsatellites and at a plumage candidate gene, the MC1R gene. Allopatric and edge populations were significantly differentiated on microsatellites, and populations were isolated by distance over the hybrid zone in Italy. Single-locus analyses showed that one locus, CmeH9, differentiated populations on different sides of the zone at the same time as showing only weak separation of populations on the same side of the zone. Within the hybrid zone there was no differentiation of phenotypes at CmeH9 or at the set of microsatellites, no excess of heterozygotes among hybrids and low levels of linkage disequilibrium between markers. We did not detect any association between phenotypes and nucleotide variation at MC1R, and the two most common haplotypes occurred in very similar frequencies in carrion and hooded crows. That we found a similar degree of genetic differentiation between allopatric and edge populations irrespectively of their location in relation to the hybrid zone, no differentiation between phenotypes within the hybrid zone, and neither heterozygote excess nor consistent linkage disequilibrium in the hybrid zone, is striking considering that carrion and hooded crows are phenotypically distinct and sometimes recognised as separate species.},
  author       = {Haas, Fredrik and Pointer, Marie A and Saino, Nicola and Brodin, Anders and Mundy, Nicholas I and Hansson, Bengt},
  issn         = {0962-1083},
  keyword      = {population structure,Corvus corone,hybridisation,Corvidae,introgression},
  language     = {eng},
  number       = {2},
  pages        = {294--305},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology},
  title        = {An analysis of population genetic differentiation and genotype-phenotype association across the hybrid zone of carrion and hooded crows using microsatellites and MC1R.},
  url          = {http://dx.doi.org/10.1111/j.1365-294X.2008.04017.x},
  volume       = {18},
  year         = {2009},
}