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Encapsulated zinc salt increases the diffusion of protein through PLG films.

Fredenberg, Susanne LU ; Reslow, Mats and Axelsson, Anders LU (2009) In International Journal of Pharmaceutics 370. p.47-53
Abstract
The use of microspheres and nanospheres of poly(d,l-lactide-co-glycolide) (PLG) as a controlled-release drug delivery system has been the subject of great interest for at least two decades within the field of pharmaceuticals. Salts of zinc and other divalent cations are sometimes co-encapsulated in PLG particles to control the pH or to stabilize encapsulated proteins or peptides. Zinc salts are known to affect pore formation and other processes that may lead to the release of an encapsulated drug. In this study the effect of encapsulated zinc acetate on protein diffusion through PLG films was investigated. PLG films, with and without encapsulated zinc acetate, were degraded in Hepes buffer for different periods of time. The films were... (More)
The use of microspheres and nanospheres of poly(d,l-lactide-co-glycolide) (PLG) as a controlled-release drug delivery system has been the subject of great interest for at least two decades within the field of pharmaceuticals. Salts of zinc and other divalent cations are sometimes co-encapsulated in PLG particles to control the pH or to stabilize encapsulated proteins or peptides. Zinc salts are known to affect pore formation and other processes that may lead to the release of an encapsulated drug. In this study the effect of encapsulated zinc acetate on protein diffusion through PLG films was investigated. PLG films, with and without encapsulated zinc acetate, were degraded in Hepes buffer for different periods of time. The films were subsequently subjected to various kinds of analyses: diffusion properties (using a diffusion cell), porosity (using scanning electron microscopy) and thickness (using light microscopy and an image-analysis program). Encapsulated zinc acetate had a considerable effect and increased the diffusion coefficient of lysozyme through PLG films degraded for 18 days or longer. Films containing zinc acetate became porous, while those without zinc acetate only developed cavities on the surface. Zinc salts may thus be used as release-modifying agents. This effect should be considered when using zinc salts as protein stabilizers or pH neutralizers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Pharmaceutics
volume
370
pages
47 - 53
publisher
Elsevier
external identifiers
  • wos:000264669600007
  • pmid:19073244
  • scopus:61349181810
ISSN
1873-3476
DOI
10.1016/j.ijpharm.2008.11.017
language
English
LU publication?
yes
id
99410695-d43a-4fda-a3eb-2cd9698f52a6 (old id 1276262)
date added to LUP
2009-01-29 15:45:28
date last changed
2017-01-01 04:28:39
@article{99410695-d43a-4fda-a3eb-2cd9698f52a6,
  abstract     = {The use of microspheres and nanospheres of poly(d,l-lactide-co-glycolide) (PLG) as a controlled-release drug delivery system has been the subject of great interest for at least two decades within the field of pharmaceuticals. Salts of zinc and other divalent cations are sometimes co-encapsulated in PLG particles to control the pH or to stabilize encapsulated proteins or peptides. Zinc salts are known to affect pore formation and other processes that may lead to the release of an encapsulated drug. In this study the effect of encapsulated zinc acetate on protein diffusion through PLG films was investigated. PLG films, with and without encapsulated zinc acetate, were degraded in Hepes buffer for different periods of time. The films were subsequently subjected to various kinds of analyses: diffusion properties (using a diffusion cell), porosity (using scanning electron microscopy) and thickness (using light microscopy and an image-analysis program). Encapsulated zinc acetate had a considerable effect and increased the diffusion coefficient of lysozyme through PLG films degraded for 18 days or longer. Films containing zinc acetate became porous, while those without zinc acetate only developed cavities on the surface. Zinc salts may thus be used as release-modifying agents. This effect should be considered when using zinc salts as protein stabilizers or pH neutralizers.},
  author       = {Fredenberg, Susanne and Reslow, Mats and Axelsson, Anders},
  issn         = {1873-3476},
  language     = {eng},
  pages        = {47--53},
  publisher    = {Elsevier},
  series       = {International Journal of Pharmaceutics},
  title        = {Encapsulated zinc salt increases the diffusion of protein through PLG films.},
  url          = {http://dx.doi.org/10.1016/j.ijpharm.2008.11.017},
  volume       = {370},
  year         = {2009},
}