Quantitative studies of the binding of the class II PapG adhesin from uropathogenic Escherichia coli to oligosaccharides.
(2003) In Bioorganic & Medicinal Chemistry 11(10). p.2255-2261- Abstract
- Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most... (More)
- Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most promising for development of improved inhibitors of PapG-mediated adhesion of E. coli. p-Methoxyphenyl galabioside was found to be most potent (Kd=140 M), and binds to PapG almost as well as the Forssman pentasaccharide. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/127945
- author
- Larsson, A ; Ohlsson, Jörgen LU ; Dodson, K W ; Hultgren, S J ; Nilsson, Ulf LU and Kihlberg, J
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioorganic & Medicinal Chemistry
- volume
- 11
- issue
- 10
- pages
- 2255 - 2261
- publisher
- Elsevier
- external identifiers
-
- wos:000182794200014
- pmid:12713835
- scopus:0344490325
- ISSN
- 0968-0896
- DOI
- 10.1016/S0968-0896(03)00114-7
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)
- id
- a23df687-bbf6-47d8-aec1-7129e2253830 (old id 127945)
- date added to LUP
- 2016-04-01 12:05:57
- date last changed
- 2022-01-26 22:46:21
@article{a23df687-bbf6-47d8-aec1-7129e2253830, abstract = {{Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most promising for development of improved inhibitors of PapG-mediated adhesion of E. coli. p-Methoxyphenyl galabioside was found to be most potent (Kd=140 M), and binds to PapG almost as well as the Forssman pentasaccharide.}}, author = {{Larsson, A and Ohlsson, Jörgen and Dodson, K W and Hultgren, S J and Nilsson, Ulf and Kihlberg, J}}, issn = {{0968-0896}}, language = {{eng}}, number = {{10}}, pages = {{2255--2261}}, publisher = {{Elsevier}}, series = {{Bioorganic & Medicinal Chemistry}}, title = {{Quantitative studies of the binding of the class II PapG adhesin from uropathogenic Escherichia coli to oligosaccharides.}}, url = {{http://dx.doi.org/10.1016/S0968-0896(03)00114-7}}, doi = {{10.1016/S0968-0896(03)00114-7}}, volume = {{11}}, year = {{2003}}, }