Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories

Wuestefeld, Anika LU orcid ; Baumeister, Hannah LU ; Adams, Jenna N ; de Flores, Robin ; Hodgetts, Carl J ; Mazloum-Farzaghi, Negar ; Olsen, Rosanna K ; Puliyadi, Vyash ; Tran, Tammy T and Bakker, Arnold , et al. (2024) In Hippocampus
Abstract

The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the entorhinal and parahippocampal cortices as well as Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the... (More)

The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the entorhinal and parahippocampal cortices as well as Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 μm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized slices spaced 5 mm apart (pixel size 0.4 μm at 20× magnification). Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while the definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed less saliently. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed neuroimaging research on the human MTL cortex.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Hippocampus
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:38415962
  • scopus:85186582782
ISSN
1050-9631
DOI
10.1002/hipo.23602
language
English
LU publication?
yes
additional info
© 2024 The Authors. Hippocampus published by Wiley Periodicals LLC.
id
127b4cf5-7c24-4cfc-9764-f64707944407
date added to LUP
2024-03-01 12:33:04
date last changed
2024-05-22 18:08:13
@article{127b4cf5-7c24-4cfc-9764-f64707944407,
  abstract     = {{<p>The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the entorhinal and parahippocampal cortices as well as Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 μm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized slices spaced 5 mm apart (pixel size 0.4 μm at 20× magnification). Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while the definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed less saliently. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed neuroimaging research on the human MTL cortex.</p>}},
  author       = {{Wuestefeld, Anika and Baumeister, Hannah and Adams, Jenna N and de Flores, Robin and Hodgetts, Carl J and Mazloum-Farzaghi, Negar and Olsen, Rosanna K and Puliyadi, Vyash and Tran, Tammy T and Bakker, Arnold and Canada, Kelsey L and Dalton, Marshall A and Daugherty, Ana M and La Joie, Renaud and Wang, Lei and Bedard, Madigan L and Buendia, Esther and Chung, Eunice and Denning, Amanda and Del Mar Arroyo-Jiménez, María and Artacho-Pérula, Emilio and Irwin, David J and Ittyerah, Ranjit and Lee, Edward B and Lim, Sydney and Del Pilar Marcos-Rabal, María and Iñiguez de Onzoño Martin, Maria Mercedes and Lopez, Monica Munoz and de la Rosa Prieto, Carlos and Schuck, Theresa and Trotman, Winifred and Vela, Alicia and Yushkevich, Paul and Amunts, Katrin and Augustinack, Jean C and Ding, Song-Lin and Insausti, Ricardo and Kedo, Olga and Berron, David and Wisse, Laura E M}},
  issn         = {{1050-9631}},
  language     = {{eng}},
  month        = {{02}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Hippocampus}},
  title        = {{Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories}},
  url          = {{http://dx.doi.org/10.1002/hipo.23602}},
  doi          = {{10.1002/hipo.23602}},
  year         = {{2024}},
}