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Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination

Muresan, Laura LU ; Valera, Ruben Ronda ; Frebort, Ivo ; Popescu, Ionel Catalin ; Csöregi, Elisabeth LU and Nistor, Mihaela LU (2008) 3rd International Workshop on Biosensors for Food Safety and Environmentsl Monitoring 163(3-4). p.219-225
Abstract
A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (-50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis... (More)
A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (-50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis (tyramine, putrescine, cadaverine, histamine, agmatine and spermidine), were investigated. Irrespective of the BA nature, the amine oxidase-based biosensor showed a linear response up to 5 mM, and its sensitivity decreases in the following order: cadaverine, putrescine, spermidine, agmatine, histamine and tyramine. The approach was used to estimate the BA content in fish samples, after their extraction with methanesulfonic acid. (Less)
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author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Food quality, Fish freshness, chromatography, Cation exchange, Amperometric biosensors, Biogenic amines
host publication
MICROCHIMICA ACTA
volume
163
issue
3-4
pages
219 - 225
publisher
Springer
conference name
3rd International Workshop on Biosensors for Food Safety and Environmentsl Monitoring
conference dates
2007-10-18 - 2007-10-20
external identifiers
  • wos:000260526300011
  • scopus:55149095371
ISSN
0026-3672
1436-5073
DOI
10.1007/s00604-008-0033-2
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
a70eec0a-9aeb-47a0-bcdb-3b7b3f524c6f (old id 1283011)
date added to LUP
2016-04-01 11:49:19
date last changed
2025-01-01 19:01:04
@inproceedings{a70eec0a-9aeb-47a0-bcdb-3b7b3f524c6f,
  abstract     = {{A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (-50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis (tyramine, putrescine, cadaverine, histamine, agmatine and spermidine), were investigated. Irrespective of the BA nature, the amine oxidase-based biosensor showed a linear response up to 5 mM, and its sensitivity decreases in the following order: cadaverine, putrescine, spermidine, agmatine, histamine and tyramine. The approach was used to estimate the BA content in fish samples, after their extraction with methanesulfonic acid.}},
  author       = {{Muresan, Laura and Valera, Ruben Ronda and Frebort, Ivo and Popescu, Ionel Catalin and Csöregi, Elisabeth and Nistor, Mihaela}},
  booktitle    = {{MICROCHIMICA ACTA}},
  issn         = {{0026-3672}},
  keywords     = {{Food quality; Fish freshness; chromatography; Cation exchange; Amperometric biosensors; Biogenic amines}},
  language     = {{eng}},
  number       = {{3-4}},
  pages        = {{219--225}},
  publisher    = {{Springer}},
  title        = {{Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination}},
  url          = {{http://dx.doi.org/10.1007/s00604-008-0033-2}},
  doi          = {{10.1007/s00604-008-0033-2}},
  volume       = {{163}},
  year         = {{2008}},
}