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The Influence of X-Rays on the Structural Studies of Peroxide-Derived Myoglobin Intermediates

Hersleth, Hans-Petter; Hsiao, Ya-Wen LU ; Ryde, Ulf LU ; Gorbitz, Carl Henrik and Andersson, K. Kristoffer (2008) In Chemistry and Biodiversity 5(10). p.2067-2089
Abstract
In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals.... (More)
In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals. In this review, the structural work and the influence of X-rays on these intermediates in myoglobin are summarized and viewed in light of analogous studies on similar intermediates in peroxidases and oxygenases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemistry and Biodiversity
volume
5
issue
10
pages
2067 - 2089
publisher
Verlag Helvetica Chimica Acta
external identifiers
  • wos:000260920000012
  • scopus:58149337266
ISSN
1612-1872
language
English
LU publication?
yes
id
1f98438b-112f-4616-9730-6adbfdd080b2 (old id 1283519)
date added to LUP
2009-02-10 11:06:38
date last changed
2017-05-21 03:34:15
@article{1f98438b-112f-4616-9730-6adbfdd080b2,
  abstract     = {In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals. In this review, the structural work and the influence of X-rays on these intermediates in myoglobin are summarized and viewed in light of analogous studies on similar intermediates in peroxidases and oxygenases.},
  author       = {Hersleth, Hans-Petter and Hsiao, Ya-Wen and Ryde, Ulf and Gorbitz, Carl Henrik and Andersson, K. Kristoffer},
  issn         = {1612-1872},
  language     = {eng},
  number       = {10},
  pages        = {2067--2089},
  publisher    = {Verlag Helvetica Chimica Acta},
  series       = {Chemistry and Biodiversity},
  title        = {The Influence of X-Rays on the Structural Studies of Peroxide-Derived Myoglobin Intermediates},
  volume       = {5},
  year         = {2008},
}