The Influence of X-Rays on the Structural Studies of Peroxide-Derived Myoglobin Intermediates
Hersleth, Hans-Petter ; Hsiao, Ya-Wen LU ; Ryde, Ulf LU
; Gorbitz, Carl Henrik
and Andersson, K. Kristoffer
(2008)
In Chemistry and Biodiversity
5(10).
p.2067-2089
- Abstract
- In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals.... (More)
- In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals. In this review, the structural work and the influence of X-rays on these intermediates in myoglobin are summarized and viewed in light of analogous studies on similar intermediates in peroxidases and oxygenases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1283519
- author
- Hersleth, Hans-Petter
; Hsiao, Ya-Wen
LU
; Ryde, Ulf
LU
; Gorbitz, Carl Henrik
and Andersson, K. Kristoffer
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chemistry and Biodiversity
- volume
- 5
- issue
- 10
- pages
- 2067 - 2089
- publisher
- Verlag Helvetica Chimica Acta
- external identifiers
-
- wos:000260920000012
- scopus:58149337266
- ISSN
- 1612-1872
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039)
- id
- 1f98438b-112f-4616-9730-6adbfdd080b2 (old id 1283519)
- date added to LUP
- 2016-04-01 11:53:42
- date last changed
- 2023-03-18 02:50:17
@article{1f98438b-112f-4616-9730-6adbfdd080b2,
abstract = {{In recent years, the awareness of potential radiation damage of metal centers in protein crystals during crystallographic data collection has received increasing attention. The radiation damage can lead to radiation-induced changes and reduction of the metal sites. One of the research fields where these concerns have been comprehensively addressed is the study of the reaction intermediates of the heme peroxidase and oxygenase reaction cycles. For both the resting states and the high-valent intermediates, the X-rays used in the structure determination have given undesired side effects through radiation-induced changes to the trapped intermediates. However, X-rays have been used to generate and trap the peroxy/hydroperoxy state in crystals. In this review, the structural work and the influence of X-rays on these intermediates in myoglobin are summarized and viewed in light of analogous studies on similar intermediates in peroxidases and oxygenases.}},
author = {{Hersleth, Hans-Petter and Hsiao, Ya-Wen and Ryde, Ulf and Gorbitz, Carl Henrik and Andersson, K. Kristoffer}},
issn = {{1612-1872}},
language = {{eng}},
number = {{10}},
pages = {{2067--2089}},
publisher = {{Verlag Helvetica Chimica Acta}},
series = {{Chemistry and Biodiversity}},
title = {{The Influence of X-Rays on the Structural Studies of Peroxide-Derived Myoglobin Intermediates}},
url = {{https://lup.lub.lu.se/search/files/136746965/113_myo0_rev.pdf}},
volume = {{5}},
year = {{2008}},
}