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Synchronized Quartz Crystal Microbalance and Nanoplasmonic Sensing of Biomolecular Recognition Reactions

Dahlin, Andreas LU ; Jönsson, Peter LU ; Jonsson, Magnus LU ; Schmid, Emanuel; Zhou, Ye and Höök, Fredrik LU (2008) In ACS Nano 2(10). p.2174-2182
Abstract
We present a method providing synchronized measurements using the two techniques: quartz crystal microbalance with dissipation (QCM-D) monitoring and localized surface plasmon resonance (LSPR). This was achieved by letting a thin gold film perforated with short-ranged ordered plasmon-active nanoholes act as one of the electrodes of a QCM-D crystal. This enabled transmission-mode optical spectroscopy to be used to temporally resolve colorimetric changes of the LSPR active substrate induced upon bionnolecular binding events. The LSPR response could thus be compared with simultaneously obtained changes in resonance frequency, Delta f, and energy dissipation, AD, of the QCM-D device. Since the LSPR technique is preferentially sensitive to... (More)
We present a method providing synchronized measurements using the two techniques: quartz crystal microbalance with dissipation (QCM-D) monitoring and localized surface plasmon resonance (LSPR). This was achieved by letting a thin gold film perforated with short-ranged ordered plasmon-active nanoholes act as one of the electrodes of a QCM-D crystal. This enabled transmission-mode optical spectroscopy to be used to temporally resolve colorimetric changes of the LSPR active substrate induced upon bionnolecular binding events. The LSPR response could thus be compared with simultaneously obtained changes in resonance frequency, Delta f, and energy dissipation, AD, of the QCM-D device. Since the LSPR technique is preferentially sensitive to changes within the voids of the nanoholes, while the QCM-D technique is preferentially sensitive to reactions on the planar region between the holes, a surface chemistry providing the same binding kinetics on both gold and silica was used. This was achieved by coating the substrate with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), which was shown to bind in the same manner on silica and gold modified with a carboxyl-terminated thiol. In this way, the combined setup provided new information about structural changes upon PLL-g-PEG adsorption. We also demonstrate subsequent binding of NeutrAvidin and an immunoreaction utilizing biotin-modified IgG. The combined information from the synchronized measurements was also used in a new way to estimate the sensing volume of the LSPR sensor. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
apertures, immunoreaction, resonance, localized surface plasmon, biosensor, quartz crystal microbalance, ethyleneglycol, avidin
in
ACS Nano
volume
2
issue
10
pages
2174 - 2182
publisher
The American Chemical Society
external identifiers
  • wos:000260503100027
  • scopus:56849130979
ISSN
1936-086X
DOI
10.1021/nn800254h
language
English
LU publication?
yes
id
fb232004-8413-4303-b11a-a44111a564b6 (old id 1283736)
date added to LUP
2009-02-10 09:58:23
date last changed
2017-08-27 04:13:55
@article{fb232004-8413-4303-b11a-a44111a564b6,
  abstract     = {We present a method providing synchronized measurements using the two techniques: quartz crystal microbalance with dissipation (QCM-D) monitoring and localized surface plasmon resonance (LSPR). This was achieved by letting a thin gold film perforated with short-ranged ordered plasmon-active nanoholes act as one of the electrodes of a QCM-D crystal. This enabled transmission-mode optical spectroscopy to be used to temporally resolve colorimetric changes of the LSPR active substrate induced upon bionnolecular binding events. The LSPR response could thus be compared with simultaneously obtained changes in resonance frequency, Delta f, and energy dissipation, AD, of the QCM-D device. Since the LSPR technique is preferentially sensitive to changes within the voids of the nanoholes, while the QCM-D technique is preferentially sensitive to reactions on the planar region between the holes, a surface chemistry providing the same binding kinetics on both gold and silica was used. This was achieved by coating the substrate with poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), which was shown to bind in the same manner on silica and gold modified with a carboxyl-terminated thiol. In this way, the combined setup provided new information about structural changes upon PLL-g-PEG adsorption. We also demonstrate subsequent binding of NeutrAvidin and an immunoreaction utilizing biotin-modified IgG. The combined information from the synchronized measurements was also used in a new way to estimate the sensing volume of the LSPR sensor.},
  author       = {Dahlin, Andreas and Jönsson, Peter and Jonsson, Magnus and Schmid, Emanuel and Zhou, Ye and Höök, Fredrik},
  issn         = {1936-086X},
  keyword      = {apertures,immunoreaction,resonance,localized surface plasmon,biosensor,quartz crystal microbalance,ethyleneglycol,avidin},
  language     = {eng},
  number       = {10},
  pages        = {2174--2182},
  publisher    = {The American Chemical Society},
  series       = {ACS Nano},
  title        = {Synchronized Quartz Crystal Microbalance and Nanoplasmonic Sensing of Biomolecular Recognition Reactions},
  url          = {http://dx.doi.org/10.1021/nn800254h},
  volume       = {2},
  year         = {2008},
}