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Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture

Sekula, Sylwia ; Fuchs, Jeanette ; Weg-Remers, Susanne ; Nagel, Peter ; Schuppler, Stefan ; Fragala, Joe ; Theilacker, Nora ; Franueb, Matthias ; Wingren, Christer LU and Ellmark, Peter LU , et al. (2008) In Small 4(10). p.1785-1793
Abstract
Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured... (More)
Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteomics, phospholipids, dip-pen nanolithography, biomimetics, cell adhesion
in
Small
volume
4
issue
10
pages
1785 - 1793
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000260201700035
  • scopus:55349114734
ISSN
1613-6829
DOI
10.1002/smll.200800949
language
English
LU publication?
yes
id
5c0abfc2-e813-44c0-8449-75edc635d82b (old id 1284097)
date added to LUP
2016-04-01 11:43:13
date last changed
2025-04-04 14:21:22
@article{5c0abfc2-e813-44c0-8449-75edc635d82b,
  abstract     = {{Molecular patterning processes taking place in biological systems are challenging to study in vivo because of their dynamic behavior, subcellular size, and high degree of complexity. In vitro patterning of biomolecules using nanolithography allows simplification of the processes and detailed study of the dynamic interactions. Parallel dip-pen nanolithography (DPN) is uniquely capable of integrating functional biomolecules on subcellular length scales due to its constructive nature, high resolution, and high throughput. Phospholipids are particularly well suited as inks for DPN since a variety of different functional lipids can be readily patterned in parallel. Here DPN is used to spatially pattern multicomponent micro- and nano-structured supported lipid membranes and multilayers that are fluid and contain various amounts of biotin and/or nitrilotriacetic acid functional groups. The patterns are characterized by fluorescence microscopy and photoemission electron microscopy. Selective adsorption of functionalized or recombinant proteins based on streptavidin or histidine-tag coupling enables the semisynthetic fabrication of model peripheral membrane bound proteins. The biomimetic membrane patterns formed in this way are then used as substrates for cell culture, as demonstrated by the selective adhesion and activation of T-cells.}},
  author       = {{Sekula, Sylwia and Fuchs, Jeanette and Weg-Remers, Susanne and Nagel, Peter and Schuppler, Stefan and Fragala, Joe and Theilacker, Nora and Franueb, Matthias and Wingren, Christer and Ellmark, Peter and Borrebaeck, Carl and Mirkin, Chad A. and Fuchs, Harald and Lenhert, Steven}},
  issn         = {{1613-6829}},
  keywords     = {{proteomics; phospholipids; dip-pen nanolithography; biomimetics; cell adhesion}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1785--1793}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Small}},
  title        = {{Multiplexed Lipid Dip-Pen Nanolithography on Subcellular Scales for the Templating of Functional Proteins and Cell Culture}},
  url          = {{http://dx.doi.org/10.1002/smll.200800949}},
  doi          = {{10.1002/smll.200800949}},
  volume       = {{4}},
  year         = {{2008}},
}