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Staphylococcal enterotoxin H induces V alpha-specific expansion of T cells.

Lindkvist, Karin LU ; Pettersson, Helen; Skartved, Niels Jörgen; Walse, Björn and Forsberg, Göran (2003) In Journal of Immunology 170(8). p.4148-4154
Abstract
Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties... (More)
Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties that fit well with V reactivity. As SEH binds on top of MHC class II, an interaction between MHC and TCR upon SEH binding is not likely. This concludes that the specific expansion of TCR V is not due to contacts between MHC and TCR, instead we suggest that SEH directly interacts with the TCR V domain. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
170
issue
8
pages
4148 - 4154
publisher
American Association of Immunologists
external identifiers
  • pmid:12682246
  • wos:000182171100027
ISSN
1550-6606
language
English
LU publication?
yes
id
5fb52653-3d60-4247-8abf-3cb9e48f9fc8 (old id 128521)
alternative location
http://www.jimmunol.org/cgi/content/abstract/170/8/4148
date added to LUP
2007-07-09 16:14:15
date last changed
2016-04-16 03:37:14
@article{5fb52653-3d60-4247-8abf-3cb9e48f9fc8,
  abstract     = {Staphylococcal enterotoxin H (SEH) is a bacterial superantigen secreted by Staphylococcus aureus. Superantigens are presented on the MHC class II and activate large amounts of T cells by cross-linking APC and T cells. In this study, RT-PCR was used to show that SEH stimulates human T cells via the V domain of TCR, in particular V10 (TRAV27), while no TCR V-specific expansion was seen. This is in sharp contrast to all other studied bacterial superantigens, which are highly specific for TCR V. It was further confirmed by flow cytometry that SEH stimulation does not alter the levels of certain TCR V. In a functional assay addressing cross-reactivity, V binding superantigens were found to form one group, whereas SEH has different properties that fit well with V reactivity. As SEH binds on top of MHC class II, an interaction between MHC and TCR upon SEH binding is not likely. This concludes that the specific expansion of TCR V is not due to contacts between MHC and TCR, instead we suggest that SEH directly interacts with the TCR V domain.},
  author       = {Lindkvist, Karin and Pettersson, Helen and Skartved, Niels Jörgen and Walse, Björn and Forsberg, Göran},
  issn         = {1550-6606},
  language     = {eng},
  number       = {8},
  pages        = {4148--4154},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Staphylococcal enterotoxin H induces V alpha-specific expansion of T cells.},
  volume       = {170},
  year         = {2003},
}