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TNF-alpha gene polymorphism and fetal Doppler velocimetry in intrauterine growth restriction

Seremak-Mrozikiewicz, Agnieszka; Dubiel, Mariusz; Drews, Krzysztof; Gudmundsson, Saemundur LU and Mrozikiewicz, Przemyslaw M. (2008) In Neuroendocrinology Letters 29(4). p.493-499
Abstract
OBJECTIVES: The intrauterine growth restriction (IUGR) frequently is a cause of fetal morbidity and mortality, and influences perinatal outcome. Several genes have been identified to explain aetiology of IUGR, beside others the gene coding for tumour necrosis factor alpha (TNF-alpha). DESIGN: To investigate frequency of AM polymorphism of TNF-alpha gene and its correlation with TNF-alpha level in maternal serum, Doppler velocimetry and perinatal outcome in pregnancies suspected for IUGR. SETTING: 42 pregnancies with IUGR and 50 matched healthy pregnant women were included in the study. Maternal venous blood samples were investigated in relationship to blood flow Doppler velocimetry in umbilical (UA) and middle cerebral (MCA) arteries. AlwI... (More)
OBJECTIVES: The intrauterine growth restriction (IUGR) frequently is a cause of fetal morbidity and mortality, and influences perinatal outcome. Several genes have been identified to explain aetiology of IUGR, beside others the gene coding for tumour necrosis factor alpha (TNF-alpha). DESIGN: To investigate frequency of AM polymorphism of TNF-alpha gene and its correlation with TNF-alpha level in maternal serum, Doppler velocimetry and perinatal outcome in pregnancies suspected for IUGR. SETTING: 42 pregnancies with IUGR and 50 matched healthy pregnant women were included in the study. Maternal venous blood samples were investigated in relationship to blood flow Doppler velocimetry in umbilical (UA) and middle cerebral (MCA) arteries. AlwI polymorphism was analysed using PCR/RFLP assays. TNF-alpha level was evaluated by immunoelectophoretic method. RESULTS: A higher frequency of mutated -238A alleles (13.1% vs. 7.0%) and genotypes containing at least one mutated -238A allele (23.8% vs. 14.0%) were found in the IUGR group. The tendency to the higher TNF-alpha level in IUGR subgroups with the presence of at least one mutated A allele (258.9 +/- 231.3 vs. 174.1 +/- 145.6 pg/ml) was detected. No statistical differences were detected for PI values in UA and MCA arteries considering particular genotypes (GG vs. GA + AA) separately in IUGR group. CONCLUSION: Increased UA vascular impedance and signs of brain sparing in MCA are related to IUGR and increased TNF-alpha level in maternal serum. AlwI polymorphism might play a role in IUGR aetiology and influence TNF-alpha expression in maternal serum, but was not related to Doppler velocimetry or with perinatal outcome. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
genetic, intrauterine growth restriction, tumour necrosis factor alpha, polymorphism, Doppler velocimetry
in
Neuroendocrinology Letters
volume
29
issue
4
pages
493 - 499
publisher
Society of Integrated Sciences
external identifiers
  • wos:000259774200019
ISSN
0172-780X
language
English
LU publication?
yes
id
d1b7b2d1-54b3-4e36-80b7-e245f56d1737 (old id 1285672)
date added to LUP
2009-02-06 09:31:32
date last changed
2016-04-15 21:25:13
@article{d1b7b2d1-54b3-4e36-80b7-e245f56d1737,
  abstract     = {OBJECTIVES: The intrauterine growth restriction (IUGR) frequently is a cause of fetal morbidity and mortality, and influences perinatal outcome. Several genes have been identified to explain aetiology of IUGR, beside others the gene coding for tumour necrosis factor alpha (TNF-alpha). DESIGN: To investigate frequency of AM polymorphism of TNF-alpha gene and its correlation with TNF-alpha level in maternal serum, Doppler velocimetry and perinatal outcome in pregnancies suspected for IUGR. SETTING: 42 pregnancies with IUGR and 50 matched healthy pregnant women were included in the study. Maternal venous blood samples were investigated in relationship to blood flow Doppler velocimetry in umbilical (UA) and middle cerebral (MCA) arteries. AlwI polymorphism was analysed using PCR/RFLP assays. TNF-alpha level was evaluated by immunoelectophoretic method. RESULTS: A higher frequency of mutated -238A alleles (13.1% vs. 7.0%) and genotypes containing at least one mutated -238A allele (23.8% vs. 14.0%) were found in the IUGR group. The tendency to the higher TNF-alpha level in IUGR subgroups with the presence of at least one mutated A allele (258.9 +/- 231.3 vs. 174.1 +/- 145.6 pg/ml) was detected. No statistical differences were detected for PI values in UA and MCA arteries considering particular genotypes (GG vs. GA + AA) separately in IUGR group. CONCLUSION: Increased UA vascular impedance and signs of brain sparing in MCA are related to IUGR and increased TNF-alpha level in maternal serum. AlwI polymorphism might play a role in IUGR aetiology and influence TNF-alpha expression in maternal serum, but was not related to Doppler velocimetry or with perinatal outcome.},
  author       = {Seremak-Mrozikiewicz, Agnieszka and Dubiel, Mariusz and Drews, Krzysztof and Gudmundsson, Saemundur and Mrozikiewicz, Przemyslaw M.},
  issn         = {0172-780X},
  keyword      = {genetic,intrauterine growth restriction,tumour necrosis factor alpha,polymorphism,Doppler velocimetry},
  language     = {eng},
  number       = {4},
  pages        = {493--499},
  publisher    = {Society of Integrated Sciences},
  series       = {Neuroendocrinology Letters},
  title        = {TNF-alpha gene polymorphism and fetal Doppler velocimetry in intrauterine growth restriction},
  volume       = {29},
  year         = {2008},
}