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Rod-Shaped Monocytes Patrol the Brain Vasculature and Give Rise to Perivascular Macrophages under the Influence of Proinflammatory Cytokines and Angiopoietin-2

Audoy-Remus, Julie; Richard, Jean-Francois; Soulet, Denis LU ; Zhou, Hong; Kubes, Paul and Vallieres, Luc (2008) In Journal of Neuroscience 28(41). p.10187-10199
Abstract
The nervous system is constantly infiltrated by blood-derived sentinels known as perivascular macrophages. Their immediate precursors have not yet been identified in situ and the mechanism that governs their recruitment is mostly unknown. Here, we provide evidence that CD68 (+)GR(-) monocytes can give rise to perivascular macrophages in mice suffering from endotoxemia. After adhesion to the endothelium, these monocytes start to crawl, adopt a rod-shaped morphology when passing through capillaries, and can manifest the ability to proliferate and form a long cytoplasmic protuberance. They are attracted in greater numbers during endotoxemia by a combination of vasoregulatory molecules, including TNF (tumor necrosis factor), interleukin-1... (More)
The nervous system is constantly infiltrated by blood-derived sentinels known as perivascular macrophages. Their immediate precursors have not yet been identified in situ and the mechanism that governs their recruitment is mostly unknown. Here, we provide evidence that CD68 (+)GR(-) monocytes can give rise to perivascular macrophages in mice suffering from endotoxemia. After adhesion to the endothelium, these monocytes start to crawl, adopt a rod-shaped morphology when passing through capillaries, and can manifest the ability to proliferate and form a long cytoplasmic protuberance. They are attracted in greater numbers during endotoxemia by a combination of vasoregulatory molecules, including TNF (tumor necrosis factor), interleukin-1 beta, and angiopoietin-2. After a period of several hours, some of them cross the endothelium to expand the population of perivascular macrophages. Depletion of adherent monocytes and perivascular macrophages can be achieved by injection of anti-angiopoietin-2 peptide-Fc fusion protein. This study extends our understanding of the behavior of monocytes at the blood-brain interface and provides a way to block their infiltration into the nervous tissue under inflammatory conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
blood-brain, endothelial, microglia, neuroinflammation, macrophage, barrier, cytokine
in
Journal of Neuroscience
volume
28
issue
41
pages
10187 - 10199
publisher
Society for Neuroscience
external identifiers
  • wos:000259912400002
  • scopus:55249088143
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.3510-08.2008
language
English
LU publication?
yes
id
beaa6366-f0bf-4dfe-bb40-302efc32cf72 (old id 1285709)
date added to LUP
2009-02-06 10:35:02
date last changed
2017-09-03 04:21:33
@article{beaa6366-f0bf-4dfe-bb40-302efc32cf72,
  abstract     = {The nervous system is constantly infiltrated by blood-derived sentinels known as perivascular macrophages. Their immediate precursors have not yet been identified in situ and the mechanism that governs their recruitment is mostly unknown. Here, we provide evidence that CD68 (+)GR(-) monocytes can give rise to perivascular macrophages in mice suffering from endotoxemia. After adhesion to the endothelium, these monocytes start to crawl, adopt a rod-shaped morphology when passing through capillaries, and can manifest the ability to proliferate and form a long cytoplasmic protuberance. They are attracted in greater numbers during endotoxemia by a combination of vasoregulatory molecules, including TNF (tumor necrosis factor), interleukin-1 beta, and angiopoietin-2. After a period of several hours, some of them cross the endothelium to expand the population of perivascular macrophages. Depletion of adherent monocytes and perivascular macrophages can be achieved by injection of anti-angiopoietin-2 peptide-Fc fusion protein. This study extends our understanding of the behavior of monocytes at the blood-brain interface and provides a way to block their infiltration into the nervous tissue under inflammatory conditions.},
  author       = {Audoy-Remus, Julie and Richard, Jean-Francois and Soulet, Denis and Zhou, Hong and Kubes, Paul and Vallieres, Luc},
  issn         = {1529-2401},
  keyword      = {blood-brain,endothelial,microglia,neuroinflammation,macrophage,barrier,cytokine},
  language     = {eng},
  number       = {41},
  pages        = {10187--10199},
  publisher    = {Society for Neuroscience},
  series       = {Journal of Neuroscience},
  title        = {Rod-Shaped Monocytes Patrol the Brain Vasculature and Give Rise to Perivascular Macrophages under the Influence of Proinflammatory Cytokines and Angiopoietin-2},
  url          = {http://dx.doi.org/10.1523/JNEUROSCI.3510-08.2008},
  volume       = {28},
  year         = {2008},
}