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A Novel Selection System for Potato Transformation Using a Mutated AHAS Gene.

Andersson, M; Trifonova, A; Andersson, A B; Johansson, M; Bülow, Leif LU and Hofvander, P (2003) In Plant Cell Reports 22(4). p.261-267
Abstract
Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a -glucuronidase (GUS) gene. Selection on 0.5 M Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with... (More)
Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a -glucuronidase (GUS) gene. Selection on 0.5 M Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Plant Cell Reports
volume
22
issue
4
pages
261 - 267
publisher
Springer
external identifiers
  • wos:000186202200005
  • pmid:14586551
  • scopus:0345581453
ISSN
1432-203X
DOI
language
English
LU publication?
yes
id
4799a188-3dbe-4ced-9930-a153c9b63ba0 (old id 128596)
date added to LUP
2007-07-16 08:22:36
date last changed
2018-06-10 03:51:26
@article{4799a188-3dbe-4ced-9930-a153c9b63ba0,
  abstract     = {Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a -glucuronidase (GUS) gene. Selection on 0.5 M Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.},
  author       = {Andersson, M and Trifonova, A and Andersson, A B and Johansson, M and Bülow, Leif and Hofvander, P},
  issn         = {1432-203X},
  language     = {eng},
  number       = {4},
  pages        = {261--267},
  publisher    = {Springer},
  series       = {Plant Cell Reports},
  title        = {A Novel Selection System for Potato Transformation Using a Mutated AHAS Gene.},
  url          = {http://dx.doi.org/},
  volume       = {22},
  year         = {2003},
}