Small angle neutron scattering of native and reconstituted silk fibroin
(2010) In Soft Matter 6(18). p.4389-4395- Abstract
Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage... (More)
Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage and spinning) of native silks.
(Less)
- author
- Greving, Imke ; Dicko, Cedric LU ; Terry, Ann LU ; Callow, Phil and Vollrath, Fritz
- publishing date
- 2010-09-21
- type
- Contribution to journal
- publication status
- published
- in
- Soft Matter
- volume
- 6
- issue
- 18
- pages
- 7 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:79951630793
- ISSN
- 1744-683X
- DOI
- 10.1039/c0sm00108b
- language
- English
- LU publication?
- no
- id
- 1285b10e-447b-4a84-a04c-65135b054ec1
- date added to LUP
- 2019-06-28 00:22:44
- date last changed
- 2022-04-10 19:17:29
@article{1285b10e-447b-4a84-a04c-65135b054ec1, abstract = {{<p>Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage and spinning) of native silks.</p>}}, author = {{Greving, Imke and Dicko, Cedric and Terry, Ann and Callow, Phil and Vollrath, Fritz}}, issn = {{1744-683X}}, language = {{eng}}, month = {{09}}, number = {{18}}, pages = {{4389--4395}}, publisher = {{Royal Society of Chemistry}}, series = {{Soft Matter}}, title = {{Small angle neutron scattering of native and reconstituted silk fibroin}}, url = {{http://dx.doi.org/10.1039/c0sm00108b}}, doi = {{10.1039/c0sm00108b}}, volume = {{6}}, year = {{2010}}, }