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Small angle neutron scattering of native and reconstituted silk fibroin

Greving, Imke ; Dicko, Cedric LU orcid ; Terry, Ann LU ; Callow, Phil and Vollrath, Fritz (2010) In Soft Matter 6(18). p.4389-4395
Abstract

Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage... (More)

Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage and spinning) of native silks.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Soft Matter
volume
6
issue
18
pages
7 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:79951630793
ISSN
1744-683X
DOI
10.1039/c0sm00108b
language
English
LU publication?
no
id
1285b10e-447b-4a84-a04c-65135b054ec1
date added to LUP
2019-06-28 00:22:44
date last changed
2022-04-10 19:17:29
@article{1285b10e-447b-4a84-a04c-65135b054ec1,
  abstract     = {{<p>Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage and spinning) of native silks.</p>}},
  author       = {{Greving, Imke and Dicko, Cedric and Terry, Ann and Callow, Phil and Vollrath, Fritz}},
  issn         = {{1744-683X}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{18}},
  pages        = {{4389--4395}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Soft Matter}},
  title        = {{Small angle neutron scattering of native and reconstituted silk fibroin}},
  url          = {{http://dx.doi.org/10.1039/c0sm00108b}},
  doi          = {{10.1039/c0sm00108b}},
  volume       = {{6}},
  year         = {{2010}},
}