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Improved Lectin-Mediated Immobilization of Human Red Blood Cells in Superporous Agarose Beads.

Gottschalk, I; Gustavsson, Per-Erik LU ; Ersson, B and Lundahl, P (2003) In Journal of Chromatography. B 784(1). p.203-208
Abstract
A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Wheat germ agglutinin, Cytochalasin B, GLUT1
in
Journal of Chromatography. B
volume
784
issue
1
pages
203 - 208
publisher
Elsevier
external identifiers
  • wos:000180412800023
  • pmid:12504199
  • scopus:0347928849
ISSN
1873-376X
DOI
10.1016/S1570-0232(02)00758-4
language
English
LU publication?
yes
id
67b75e41-70f9-42e6-9886-370c048ed213 (old id 128607)
date added to LUP
2007-07-16 09:37:23
date last changed
2018-01-07 05:08:52
@article{67b75e41-70f9-42e6-9886-370c048ed213,
  abstract     = {A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.},
  author       = {Gottschalk, I and Gustavsson, Per-Erik and Ersson, B and Lundahl, P},
  issn         = {1873-376X},
  keyword      = {Wheat germ agglutinin,Cytochalasin B,GLUT1},
  language     = {eng},
  number       = {1},
  pages        = {203--208},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Improved Lectin-Mediated Immobilization of Human Red Blood Cells in Superporous Agarose Beads.},
  url          = {http://dx.doi.org/10.1016/S1570-0232(02)00758-4},
  volume       = {784},
  year         = {2003},
}