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Bioelectrocatalytical detection of H2O2 with different forms of horseradish peroxidase directly adsorbed at polycrystalline silver and gold

Ferapontova, Elena LU and Gorton, Lo LU (2003) In Electroanalysis 15(5-6). p.484-491
Abstract
The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to... (More)
The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to NHisrHRP are analyzed and discussed, specifically, the high ET rates obtained with NHisrHRP-modified preoxidized gold and silver electrodes, though accompanied by extremely rapid loss of the biolectrocatalytical activity of the latter (silver) in direct (but not mediated) ET. The advantages and drawbacks of the studied HRP-electrode systems for the electroanalytical detection of H2O2 are considered. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Polycrystalline gold, Polycrystalline silver, Heterogeneous direct electron transfer, Histidine, Horseradish peroxidase, Bioelectrocatalysis
in
Electroanalysis
volume
15
issue
5-6
pages
484 - 491
publisher
John Wiley & Sons
external identifiers
  • wos:000182694800020
  • scopus:0037997920
ISSN
1040-0397
DOI
language
English
LU publication?
yes
id
4a56c9c9-48b6-4d3b-b078-c552f8dad7f8 (old id 128899)
date added to LUP
2007-06-26 15:56:52
date last changed
2018-05-29 12:28:07
@article{4a56c9c9-48b6-4d3b-b078-c552f8dad7f8,
  abstract     = {The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to NHisrHRP are analyzed and discussed, specifically, the high ET rates obtained with NHisrHRP-modified preoxidized gold and silver electrodes, though accompanied by extremely rapid loss of the biolectrocatalytical activity of the latter (silver) in direct (but not mediated) ET. The advantages and drawbacks of the studied HRP-electrode systems for the electroanalytical detection of H2O2 are considered.},
  author       = {Ferapontova, Elena and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {Polycrystalline gold,Polycrystalline silver,Heterogeneous direct electron transfer,Histidine,Horseradish peroxidase,Bioelectrocatalysis},
  language     = {eng},
  number       = {5-6},
  pages        = {484--491},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Bioelectrocatalytical detection of H2O2 with different forms of horseradish peroxidase directly adsorbed at polycrystalline silver and gold},
  url          = {http://dx.doi.org/},
  volume       = {15},
  year         = {2003},
}