Bioelectrocatalytical detection of H2O2 with different forms of horseradish peroxidase directly adsorbed at polycrystalline silver and gold
(2003) In Electroanalysis 15(5-6). p.484-491- Abstract
- The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to... (More)
- The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to NHisrHRP are analyzed and discussed, specifically, the high ET rates obtained with NHisrHRP-modified preoxidized gold and silver electrodes, though accompanied by extremely rapid loss of the biolectrocatalytical activity of the latter (silver) in direct (but not mediated) ET. The advantages and drawbacks of the studied HRP-electrode systems for the electroanalytical detection of H2O2 are considered. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128899
- author
- Ferapontova, Elena LU and Gorton, Lo LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Polycrystalline gold, Polycrystalline silver, Heterogeneous direct electron transfer, Histidine, Horseradish peroxidase, Bioelectrocatalysis
- in
- Electroanalysis
- volume
- 15
- issue
- 5-6
- pages
- 484 - 491
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000182694800020
- scopus:0037997920
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.200390058
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 4a56c9c9-48b6-4d3b-b078-c552f8dad7f8 (old id 128899)
- date added to LUP
- 2016-04-01 16:41:03
- date last changed
- 2022-01-28 21:23:11
@article{4a56c9c9-48b6-4d3b-b078-c552f8dad7f8, abstract = {{The bioelectrocatalytical reduction of H2O2 based on direct electron transfer (ET) between polycrystalline silver and the heme containing active site of horseradish peroxidase (HRP) is studied and compared with that obtained at gold. Native HRP and recombinant wild type HRP, containing additionally a six-histidine tag at the N-terminus, NHisrHRP, have been used for adsorptive modification of the electrodes. The histidine sequences, introduced into the peroxidase structure by genetic engineering of recombinant HRP using an E. coli expression system, were supposed to affect adsorption/orientation of the enzyme at the electrode surface. The variations in direct ET efficiency when changing from gold to silver, as well as from native HRP to NHisrHRP are analyzed and discussed, specifically, the high ET rates obtained with NHisrHRP-modified preoxidized gold and silver electrodes, though accompanied by extremely rapid loss of the biolectrocatalytical activity of the latter (silver) in direct (but not mediated) ET. The advantages and drawbacks of the studied HRP-electrode systems for the electroanalytical detection of H2O2 are considered.}}, author = {{Ferapontova, Elena and Gorton, Lo}}, issn = {{1040-0397}}, keywords = {{Polycrystalline gold; Polycrystalline silver; Heterogeneous direct electron transfer; Histidine; Horseradish peroxidase; Bioelectrocatalysis}}, language = {{eng}}, number = {{5-6}}, pages = {{484--491}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electroanalysis}}, title = {{Bioelectrocatalytical detection of H2O2 with different forms of horseradish peroxidase directly adsorbed at polycrystalline silver and gold}}, url = {{http://dx.doi.org/10.1002/elan.200390058}}, doi = {{10.1002/elan.200390058}}, volume = {{15}}, year = {{2003}}, }