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Characterization of graphite electrodes modified with laccase from Trametes versicolor and their use for bioelectrochemical monitoring of phenolic compounds in flow injection analysis

Haghighi, Behzad LU ; Gorton, Lo LU ; Ruzgas, Tautgirdas LU and Jönsson, L J (2003) In Analytica Chimica Acta 487(1). p.3-14
Abstract
Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working... (More)
Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working potential of −50 mV versus Ag|AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from Coriolus hirsutus, cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium and horseradish peroxidase (HRP). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phenols, Laccase, Amperometry, Flow injection, Biosensors
in
Analytica Chimica Acta
volume
487
issue
1
pages
3 - 14
publisher
Elsevier
external identifiers
  • wos:000183777500002
  • scopus:0037669001
ISSN
1873-4324
DOI
language
English
LU publication?
yes
id
c2b0facb-ff51-480d-9a58-ef807a748b02 (old id 128913)
date added to LUP
2007-06-27 08:38:45
date last changed
2018-06-17 04:41:57
@article{c2b0facb-ff51-480d-9a58-ef807a748b02,
  abstract     = {Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 M, n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution ( at pH 5.0), flow rate of 0.51 ml min−1 and a working potential of −50 mV versus Ag|AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from Coriolus hirsutus, cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium and horseradish peroxidase (HRP).},
  author       = {Haghighi, Behzad and Gorton, Lo and Ruzgas, Tautgirdas and Jönsson, L J},
  issn         = {1873-4324},
  keyword      = {Phenols,Laccase,Amperometry,Flow injection,Biosensors},
  language     = {eng},
  number       = {1},
  pages        = {3--14},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Characterization of graphite electrodes modified with laccase from Trametes versicolor and their use for bioelectrochemical monitoring of phenolic compounds in flow injection analysis},
  url          = {http://dx.doi.org/},
  volume       = {487},
  year         = {2003},
}