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Electrochemical and catalytic investigation of carbon paste modified with Toluidine Blue O covalently immobilised on silica gel

Munteanu, Florentina LU ; Okamoto, Yoshiyuki and Gorton, Lo LU (2003) In Analytica Chimica Acta 476(1). p.43-54
Abstract
Toluidine Blue O (TBO) was covalently bound on silica gel and mixed with graphite powder and paraffin oil to produce modified carbon paste electrodes. The formal potential (E°′) of the covalently bound TBO was found to be −100 mV versus Ag|AgCl (KCl sat.) at pH 7.0 and the E°′ varied less than anticipated for a 2-electron–proton type mediator with pH. The bound TBO was found to act as an efficient electron acceptor for NADH as well as electron donor for oxidised horseradish peroxidase (HRP). The kinetics and the mechanism of the reaction between NADH and TBO were investigated with cyclic voltammetry and using a rotating disc electrode. Further experiments were done in the flow injection mode injecting different concentrations of NADH.... (More)
Toluidine Blue O (TBO) was covalently bound on silica gel and mixed with graphite powder and paraffin oil to produce modified carbon paste electrodes. The formal potential (E°′) of the covalently bound TBO was found to be −100 mV versus Ag|AgCl (KCl sat.) at pH 7.0 and the E°′ varied less than anticipated for a 2-electron–proton type mediator with pH. The bound TBO was found to act as an efficient electron acceptor for NADH as well as electron donor for oxidised horseradish peroxidase (HRP). The kinetics and the mechanism of the reaction between NADH and TBO were investigated with cyclic voltammetry and using a rotating disc electrode. Further experiments were done in the flow injection mode injecting different concentrations of NADH. Similar studies were done in the presence of hydrogen peroxide when HRP was adsorbed onto the TBO modified silica gel carbon paste electrodes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
476
issue
1
pages
43 - 54
publisher
Elsevier
external identifiers
  • wos:000180168800005
  • scopus:0037427650
ISSN
1873-4324
DOI
10.1016/S0003-2670(02)01359-4
language
English
LU publication?
yes
id
88dcc128-3c5a-4d07-8f4d-c9b6d105753a (old id 128942)
date added to LUP
2007-06-27 13:13:06
date last changed
2018-07-29 03:57:15
@article{88dcc128-3c5a-4d07-8f4d-c9b6d105753a,
  abstract     = {Toluidine Blue O (TBO) was covalently bound on silica gel and mixed with graphite powder and paraffin oil to produce modified carbon paste electrodes. The formal potential (E°′) of the covalently bound TBO was found to be −100 mV versus Ag|AgCl (KCl sat.) at pH 7.0 and the E°′ varied less than anticipated for a 2-electron–proton type mediator with pH. The bound TBO was found to act as an efficient electron acceptor for NADH as well as electron donor for oxidised horseradish peroxidase (HRP). The kinetics and the mechanism of the reaction between NADH and TBO were investigated with cyclic voltammetry and using a rotating disc electrode. Further experiments were done in the flow injection mode injecting different concentrations of NADH. Similar studies were done in the presence of hydrogen peroxide when HRP was adsorbed onto the TBO modified silica gel carbon paste electrodes.},
  author       = {Munteanu, Florentina and Okamoto, Yoshiyuki and Gorton, Lo},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1},
  pages        = {43--54},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Electrochemical and catalytic investigation of carbon paste modified with Toluidine Blue O covalently immobilised on silica gel},
  url          = {http://dx.doi.org/10.1016/S0003-2670(02)01359-4},
  volume       = {476},
  year         = {2003},
}