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Silicone Membrane Equilibrator: Measuring Chemical Activity of Nonpolar Chemicals with Poly(dimethylsiloxane) Microtubes Immersed Directly in Tissue and Lipids.

Mayer, Philipp; Toräng, Lars; Glæsner, Nadia and Jönsson, Jan Åke LU (2009) In Analytical Chemistry 81(4). p.1536-1542
Abstract
The chemical activity of organic chemicals directs their diffusion and partitioning and is consequently crucial for their transport, distribution, and toxic effects. A silicone membrane equilibrator is introduced for measuring the chemical activity of nonpolar organic chemicals in lipid-rich samples: (I) A 6 m poly(dimethylsiloxane) (PDMS) microtube (300 mum i.d., 640 mum o.d.) was placed in a sample, and a sample-PDMS equilibrium was reached within 10 min for 12 polycyclic aromatic hydrocarbons (PAHs) acting as model compounds. (II) A plug of 100 muL of methanol was pushed through the tube to equilibrate it with the PDMS and thus the sample. (III) This yielded an undiluted methanol extract that was injected into a high-performance liquid... (More)
The chemical activity of organic chemicals directs their diffusion and partitioning and is consequently crucial for their transport, distribution, and toxic effects. A silicone membrane equilibrator is introduced for measuring the chemical activity of nonpolar organic chemicals in lipid-rich samples: (I) A 6 m poly(dimethylsiloxane) (PDMS) microtube (300 mum i.d., 640 mum o.d.) was placed in a sample, and a sample-PDMS equilibrium was reached within 10 min for 12 polycyclic aromatic hydrocarbons (PAHs) acting as model compounds. (II) A plug of 100 muL of methanol was pushed through the tube to equilibrate it with the PDMS and thus the sample. (III) This yielded an undiluted methanol extract that was injected into a high-performance liquid chromatograph (HPLC) with multiband fluorescence detection. Quantification limits expressed as unitless chemical activities ranged from 6 x 10(-9) to 5 x 10(-8), and relative standard deviations were from 6% to 19%. Chemical activities of PAHs in mussels from two polluted sites were measured between 10(-7) and 10(-5), and activity coefficients for PAHs in vegetable and fish oils hardly differed between oils. This method can be used for internal exposure measurements, for monitoring product safety/conformity, and process control. The method can also be applied to measure total analyte concentrations in lipid-rich samples and oils. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
81
issue
4
pages
1536 - 1542
publisher
The American Chemical Society
external identifiers
  • wos:000263319000033
  • pmid:19146462
  • scopus:64349087925
ISSN
1520-6882
DOI
10.1021/ac802261z
language
English
LU publication?
yes
id
aaf5ed00-18d0-4b7f-b104-0de723dd05f2 (old id 1289671)
date added to LUP
2009-02-18 12:17:39
date last changed
2017-08-27 03:59:46
@article{aaf5ed00-18d0-4b7f-b104-0de723dd05f2,
  abstract     = {The chemical activity of organic chemicals directs their diffusion and partitioning and is consequently crucial for their transport, distribution, and toxic effects. A silicone membrane equilibrator is introduced for measuring the chemical activity of nonpolar organic chemicals in lipid-rich samples: (I) A 6 m poly(dimethylsiloxane) (PDMS) microtube (300 mum i.d., 640 mum o.d.) was placed in a sample, and a sample-PDMS equilibrium was reached within 10 min for 12 polycyclic aromatic hydrocarbons (PAHs) acting as model compounds. (II) A plug of 100 muL of methanol was pushed through the tube to equilibrate it with the PDMS and thus the sample. (III) This yielded an undiluted methanol extract that was injected into a high-performance liquid chromatograph (HPLC) with multiband fluorescence detection. Quantification limits expressed as unitless chemical activities ranged from 6 x 10(-9) to 5 x 10(-8), and relative standard deviations were from 6% to 19%. Chemical activities of PAHs in mussels from two polluted sites were measured between 10(-7) and 10(-5), and activity coefficients for PAHs in vegetable and fish oils hardly differed between oils. This method can be used for internal exposure measurements, for monitoring product safety/conformity, and process control. The method can also be applied to measure total analyte concentrations in lipid-rich samples and oils.},
  author       = {Mayer, Philipp and Toräng, Lars and Glæsner, Nadia and Jönsson, Jan Åke},
  issn         = {1520-6882},
  language     = {eng},
  number       = {4},
  pages        = {1536--1542},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Silicone Membrane Equilibrator: Measuring Chemical Activity of Nonpolar Chemicals with Poly(dimethylsiloxane) Microtubes Immersed Directly in Tissue and Lipids.},
  url          = {http://dx.doi.org/10.1021/ac802261z},
  volume       = {81},
  year         = {2009},
}