New mechanism for amino acid influx into human epidermal Langerhans cells: L-dopa/proton counter-transport system.
(2003) In Experimental Dermatology 12(5). p.602-609- Abstract
- We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by... (More)
- We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by LCs but can rapidly be dislocated to the intercellular space by certain extracellular amino acids, i.e. LCs can profit by L-dopa in a dualistic way, altogether a remarkable biological phenomenon. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/120344
- author
- Falck, Bengt LU ; Bendsöe, Niels LU and Ronquist, Gunnar
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Experimental Dermatology
- volume
- 12
- issue
- 5
- pages
- 602 - 609
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000185198900009
- pmid:14705800
- scopus:0142106496
- ISSN
- 0906-6705
- DOI
- 10.1034/j.1600-0625.2003.00019.x
- language
- English
- LU publication?
- yes
- id
- 128d559e-8cec-4ff4-8368-2701c08cb4f6 (old id 120344)
- alternative location
- http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=14705800&dopt=Abstract
- date added to LUP
- 2016-04-01 11:32:50
- date last changed
- 2022-04-20 18:35:22
@article{128d559e-8cec-4ff4-8368-2701c08cb4f6, abstract = {{We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by LCs but can rapidly be dislocated to the intercellular space by certain extracellular amino acids, i.e. LCs can profit by L-dopa in a dualistic way, altogether a remarkable biological phenomenon.}}, author = {{Falck, Bengt and Bendsöe, Niels and Ronquist, Gunnar}}, issn = {{0906-6705}}, language = {{eng}}, number = {{5}}, pages = {{602--609}}, publisher = {{Wiley-Blackwell}}, series = {{Experimental Dermatology}}, title = {{New mechanism for amino acid influx into human epidermal Langerhans cells: L-dopa/proton counter-transport system.}}, url = {{https://lup.lub.lu.se/search/files/2538616/623944.pdf}}, doi = {{10.1034/j.1600-0625.2003.00019.x}}, volume = {{12}}, year = {{2003}}, }