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A capacitive immunosensor for detection of cholera toxin

Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU (2009) In Analytica Chimica Acta 634(2). p.255-261
Abstract
Contamination of food with biological toxins aswell as their potential use asweapons of mass destruction has created an urge for rapid and cost effective analytical techniques capable of detecting trace amounts of these toxins. This paper describes the development of a sensitive method for detection of cholera toxin (CT) using a flow-injection capacitive immunosensor based on self-assembled monolayers. The sensing surface consists of monoclonal antibodies against the B subunit of CT (anti-CT), immobilized on a gold transducer. Experimental results showthat the immunosensor responded linearly to CT concentrations in the range from 1.0×10−13 to 1.0×10−10 M under optimized conditions. The limit of detection (LOD) was 1.0×10−14 M. Two more... (More)
Contamination of food with biological toxins aswell as their potential use asweapons of mass destruction has created an urge for rapid and cost effective analytical techniques capable of detecting trace amounts of these toxins. This paper describes the development of a sensitive method for detection of cholera toxin (CT) using a flow-injection capacitive immunosensor based on self-assembled monolayers. The sensing surface consists of monoclonal antibodies against the B subunit of CT (anti-CT), immobilized on a gold transducer. Experimental results showthat the immunosensor responded linearly to CT concentrations in the range from 1.0×10−13 to 1.0×10−10 M under optimized conditions. The limit of detection (LOD) was 1.0×10−14 M. Two more analytical methods were employed for detection of CT using the same antibody namely, sandwich ELISA and surface plasmon resonance (SPR)-based immunosensor. The former had an LOD of 1.2×10−12 M and a working range from3.7×10−11 to 2.9×10−10 M whereas, the latter had an LOD of 1.0×10−11 M and a linearity ranging from 1.0×10−9 to 1.0×10−6 M. These results demonstrate that the developed capacitive immunosensor system has a higher sensitivity than the other two techniques. The binding affinity of CT to the immobilized anti-CT was determined using the SPR-based immunosensor and an association constant (KA) of 1.4×109 M−1 was estimated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Surface plasmon resonance, Cholera toxin, Capacitive immunosensor, Enzyme-linked immunosorbent assays
in
Analytica Chimica Acta
volume
634
issue
2
pages
255 - 261
publisher
Elsevier
external identifiers
  • pmid:19185129
  • wos:000263390400018
  • scopus:58849105024
ISSN
1873-4324
DOI
10.1016/j.aca.2008.12.035
language
English
LU publication?
yes
id
59bf224b-647f-4da3-a8b8-156fc22c1ba5 (old id 1290510)
date added to LUP
2009-02-05 15:56:11
date last changed
2017-11-19 03:47:21
@article{59bf224b-647f-4da3-a8b8-156fc22c1ba5,
  abstract     = {Contamination of food with biological toxins aswell as their potential use asweapons of mass destruction has created an urge for rapid and cost effective analytical techniques capable of detecting trace amounts of these toxins. This paper describes the development of a sensitive method for detection of cholera toxin (CT) using a flow-injection capacitive immunosensor based on self-assembled monolayers. The sensing surface consists of monoclonal antibodies against the B subunit of CT (anti-CT), immobilized on a gold transducer. Experimental results showthat the immunosensor responded linearly to CT concentrations in the range from 1.0×10−13 to 1.0×10−10 M under optimized conditions. The limit of detection (LOD) was 1.0×10−14 M. Two more analytical methods were employed for detection of CT using the same antibody namely, sandwich ELISA and surface plasmon resonance (SPR)-based immunosensor. The former had an LOD of 1.2×10−12 M and a working range from3.7×10−11 to 2.9×10−10 M whereas, the latter had an LOD of 1.0×10−11 M and a linearity ranging from 1.0×10−9 to 1.0×10−6 M. These results demonstrate that the developed capacitive immunosensor system has a higher sensitivity than the other two techniques. The binding affinity of CT to the immobilized anti-CT was determined using the SPR-based immunosensor and an association constant (KA) of 1.4×109 M−1 was estimated.},
  author       = {Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo},
  issn         = {1873-4324},
  keyword      = {Surface plasmon resonance,Cholera toxin,Capacitive immunosensor,Enzyme-linked immunosorbent assays},
  language     = {eng},
  number       = {2},
  pages        = {255--261},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {A capacitive immunosensor for detection of cholera toxin},
  url          = {http://dx.doi.org/10.1016/j.aca.2008.12.035},
  volume       = {634},
  year         = {2009},
}