Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection
(2003) In Biosensors & Bioelectronics 18(5-6). p.547-553- Abstract
- A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/129098
- author
- Bontidean, Ibolya LU ; Ahlqvist, Josefin LU ; Mulchandani, Ashok ; Chen, Wilfred ; Bae, Weon ; Mehra, Rajesh K ; Mortari, Alessia LU and Csöregi, Elisabeth LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biosensors, Heavy metals, Phytochelatins, Capacitance
- in
- Biosensors & Bioelectronics
- volume
- 18
- issue
- 5-6
- pages
- 547 - 553
- publisher
- Elsevier
- external identifiers
-
- wos:000182790500007
- pmid:12706561
- scopus:0037403268
- ISSN
- 1873-4235
- DOI
- 10.1016/S0956-5663(03)00026-5
- language
- English
- LU publication?
- yes
- id
- fd5edfe7-fb23-409c-aecf-0cd462692678 (old id 129098)
- date added to LUP
- 2016-04-01 16:41:39
- date last changed
- 2022-04-22 23:50:28
@article{fd5edfe7-fb23-409c-aecf-0cd462692678, abstract = {{A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.}}, author = {{Bontidean, Ibolya and Ahlqvist, Josefin and Mulchandani, Ashok and Chen, Wilfred and Bae, Weon and Mehra, Rajesh K and Mortari, Alessia and Csöregi, Elisabeth}}, issn = {{1873-4235}}, keywords = {{Biosensors; Heavy metals; Phytochelatins; Capacitance}}, language = {{eng}}, number = {{5-6}}, pages = {{547--553}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection}}, url = {{http://dx.doi.org/10.1016/S0956-5663(03)00026-5}}, doi = {{10.1016/S0956-5663(03)00026-5}}, volume = {{18}}, year = {{2003}}, }