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Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

Bontidean, Ibolya LU ; Ahlqvist, Josefin LU ; Mulchandani, Ashok; Chen, Wilfred; Bae, Weon; Mehra, Rajesh K; Mortari, Alessia LU and Csöregi, Elisabeth LU (2003) In Biosensors & Bioelectronics 18(5-6). p.547-553
Abstract
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensors, Heavy metals, Phytochelatins, Capacitance
in
Biosensors & Bioelectronics
volume
18
issue
5-6
pages
547 - 553
publisher
Elsevier
external identifiers
  • wos:000182790500007
  • pmid:12706561
  • scopus:0037403268
ISSN
1873-4235
DOI
10.1016/S0956-5663(03)00026-5
language
English
LU publication?
yes
id
fd5edfe7-fb23-409c-aecf-0cd462692678 (old id 129098)
date added to LUP
2007-07-02 08:44:30
date last changed
2018-09-16 04:16:02
@article{fd5edfe7-fb23-409c-aecf-0cd462692678,
  abstract     = {A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.},
  author       = {Bontidean, Ibolya and Ahlqvist, Josefin and Mulchandani, Ashok and Chen, Wilfred and Bae, Weon and Mehra, Rajesh K and Mortari, Alessia and Csöregi, Elisabeth},
  issn         = {1873-4235},
  keyword      = {Biosensors,Heavy metals,Phytochelatins,Capacitance},
  language     = {eng},
  number       = {5-6},
  pages        = {547--553},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection},
  url          = {http://dx.doi.org/10.1016/S0956-5663(03)00026-5},
  volume       = {18},
  year         = {2003},
}