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Integrated immobilized cell reactor–adsorption system for b-cyclodextrin production: a model study using PVA-cryogel entrapped Bacillus agaradhaerens cells.

Martins, Rita LU ; Plieva, Fatima LU ; Santos, Ana and Hatti-Kaul, Rajni LU (2003) In Biotechnology Letters 25(18). p.1537-1543
Abstract
Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to... (More)
Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to Amberlite XAD-4 in a recycle batch mode. The product adsorption was facilitated at low temperature while hot water was used for elution. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
25
issue
18
pages
1537 - 1543
publisher
Springer
external identifiers
  • wos:000184957400010
  • pmid:14571979
  • scopus:0141836934
ISSN
1573-6776
DOI
language
English
LU publication?
yes
id
1990dc1f-0a81-4cce-936b-8d42f32e5531 (old id 129225)
date added to LUP
2007-07-02 13:46:37
date last changed
2018-05-29 11:20:57
@article{1990dc1f-0a81-4cce-936b-8d42f32e5531,
  abstract     = {Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to Amberlite XAD-4 in a recycle batch mode. The product adsorption was facilitated at low temperature while hot water was used for elution.},
  author       = {Martins, Rita and Plieva, Fatima and Santos, Ana and Hatti-Kaul, Rajni},
  issn         = {1573-6776},
  language     = {eng},
  number       = {18},
  pages        = {1537--1543},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Integrated immobilized cell reactor–adsorption system for b-cyclodextrin production: a model study using PVA-cryogel entrapped Bacillus agaradhaerens cells.},
  url          = {http://dx.doi.org/},
  volume       = {25},
  year         = {2003},
}