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Isolation and characterization of water-soluble hemicelluloses from flax shive,

Jacobs, Anna; Palm, Magnus LU ; Zacchi, Guido LU and Dahlman, Olof (2003) In Carbohydrate Research 338(18). p.1869-1876
Abstract
Partially depolymerized, water-soluble hemicelluloses were solubilized from flax shive employing hydrothermal microwave treatment and thereafter subjected to ion-exchange chromatography, enzymatic purification and/or size-exclusion chromatography (SEC). The oligo- and polysaccharide fractions thus obtained were characterized with respect to molar mass, molar mass distribution, degree of polymerization (DP) and degree of substitution with acetyl moieties (DSAc) by employing SEC in combination with MALDI-TOF mass spectrometry. The major portion of the water-soluble flax hemicellulose consisted of an O-acetyl-4-O-methylglucuronoxylan exhibiting a DPp value (i.e., peak-average DP) of 28. When the DSAc for this O-acetyl-4-O-methylglucuronoxylan... (More)
Partially depolymerized, water-soluble hemicelluloses were solubilized from flax shive employing hydrothermal microwave treatment and thereafter subjected to ion-exchange chromatography, enzymatic purification and/or size-exclusion chromatography (SEC). The oligo- and polysaccharide fractions thus obtained were characterized with respect to molar mass, molar mass distribution, degree of polymerization (DP) and degree of substitution with acetyl moieties (DSAc) by employing SEC in combination with MALDI-TOF mass spectrometry. The major portion of the water-soluble flax hemicellulose consisted of an O-acetyl-4-O-methylglucuronoxylan exhibiting a DPp value (i.e., peak-average DP) of 28. When the DSAc for this O-acetyl-4-O-methylglucuronoxylan was calculated on the basis of the MALDI-MS spectra obtained without and following deacetylation, a value of 0.7 was obtained. In addition, an O-acetyl-glucomannan (DPp=9, DS=0.4) and minor quantities of small neutral O-acetyl-xylooligosaccharides were also isolated from the mixture of water-soluble hemicelluloses released from the flax shive by microwave treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Carbohydrate Research
volume
338
issue
18
pages
1869 - 1876
publisher
Elsevier
external identifiers
  • pmid:12932370
  • wos:000185122500007
  • scopus:0142026902
ISSN
1873-426X
DOI
10.1016/S0008-6215(03)00308-2
language
English
LU publication?
yes
id
169a264a-c9c7-47d6-8229-989cab03561c (old id 129325)
date added to LUP
2007-06-25 11:32:57
date last changed
2018-07-15 03:39:18
@article{169a264a-c9c7-47d6-8229-989cab03561c,
  abstract     = {Partially depolymerized, water-soluble hemicelluloses were solubilized from flax shive employing hydrothermal microwave treatment and thereafter subjected to ion-exchange chromatography, enzymatic purification and/or size-exclusion chromatography (SEC). The oligo- and polysaccharide fractions thus obtained were characterized with respect to molar mass, molar mass distribution, degree of polymerization (DP) and degree of substitution with acetyl moieties (DSAc) by employing SEC in combination with MALDI-TOF mass spectrometry. The major portion of the water-soluble flax hemicellulose consisted of an O-acetyl-4-O-methylglucuronoxylan exhibiting a DPp value (i.e., peak-average DP) of 28. When the DSAc for this O-acetyl-4-O-methylglucuronoxylan was calculated on the basis of the MALDI-MS spectra obtained without and following deacetylation, a value of 0.7 was obtained. In addition, an O-acetyl-glucomannan (DPp=9, DS=0.4) and minor quantities of small neutral O-acetyl-xylooligosaccharides were also isolated from the mixture of water-soluble hemicelluloses released from the flax shive by microwave treatment.},
  author       = {Jacobs, Anna and Palm, Magnus and Zacchi, Guido and Dahlman, Olof},
  issn         = {1873-426X},
  language     = {eng},
  number       = {18},
  pages        = {1869--1876},
  publisher    = {Elsevier},
  series       = {Carbohydrate Research},
  title        = {Isolation and characterization of water-soluble hemicelluloses from flax shive,},
  url          = {http://dx.doi.org/10.1016/S0008-6215(03)00308-2},
  volume       = {338},
  year         = {2003},
}