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Engineering of the metabolism of Saccharomyces cerevisiae for anaerobic production of mannitol,

Costenoble, R; Adler, L; Niklasson, C and Lidén, Gunnar LU (2003) In FEMS Yeast Research 3(1). p.17-25
Abstract
Under anaerobic conditions, Saccharomyces cerevisiae uses NADH-dependent glycerol-3-phosphate dehydrogenase (Gpd1p and Gpd2p) to re-oxidize excess NADH, yielding substantial amounts of glycerol. In a Δgpd1 Δgpd2 double-null mutant, the necessary NAD+ regeneration through glycerol production is no longer possible, and this mutant does not grow under anaerobic conditions. The excess NADH formed can potentially be used to drive other NADH-dependent reactions or pathways. To investigate this possibility, a double-null mutant was transformed with a heterologous gene (mtlD) from Escherichia coli, coding for NADH-dependent mannitol-1-phosphate dehydrogenase. Expression of this gene in S. cerevisiae should result in NADH oxidation by the... (More)
Under anaerobic conditions, Saccharomyces cerevisiae uses NADH-dependent glycerol-3-phosphate dehydrogenase (Gpd1p and Gpd2p) to re-oxidize excess NADH, yielding substantial amounts of glycerol. In a Δgpd1 Δgpd2 double-null mutant, the necessary NAD+ regeneration through glycerol production is no longer possible, and this mutant does not grow under anaerobic conditions. The excess NADH formed can potentially be used to drive other NADH-dependent reactions or pathways. To investigate this possibility, a double-null mutant was transformed with a heterologous gene (mtlD) from Escherichia coli, coding for NADH-dependent mannitol-1-phosphate dehydrogenase. Expression of this gene in S. cerevisiae should result in NADH oxidation by the NADH-requiring formation of mannitol-1-phosphate from fructose-6-phosphate. The strain was characterized using step-change experiments, in which, during the exponential growth phase, the inlet gas was changed from air to nitrogen. It was found that the mutant produced mannitol only under anaerobic conditions. However, anaerobic growth was not regained, which was probably due to the excessive accumulation of mannitol in the cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Yeast Research
volume
3
issue
1
pages
17 - 25
publisher
Elsevier
external identifiers
  • scopus:12244270398
ISSN
1567-1364
DOI
language
English
LU publication?
yes
id
c4ae33b3-8226-4b5b-a86f-6ac7f89c82c0 (old id 129373)
date added to LUP
2007-06-25 10:38:00
date last changed
2018-05-29 10:14:08
@article{c4ae33b3-8226-4b5b-a86f-6ac7f89c82c0,
  abstract     = {Under anaerobic conditions, Saccharomyces cerevisiae uses NADH-dependent glycerol-3-phosphate dehydrogenase (Gpd1p and Gpd2p) to re-oxidize excess NADH, yielding substantial amounts of glycerol. In a Δgpd1 Δgpd2 double-null mutant, the necessary NAD+ regeneration through glycerol production is no longer possible, and this mutant does not grow under anaerobic conditions. The excess NADH formed can potentially be used to drive other NADH-dependent reactions or pathways. To investigate this possibility, a double-null mutant was transformed with a heterologous gene (mtlD) from Escherichia coli, coding for NADH-dependent mannitol-1-phosphate dehydrogenase. Expression of this gene in S. cerevisiae should result in NADH oxidation by the NADH-requiring formation of mannitol-1-phosphate from fructose-6-phosphate. The strain was characterized using step-change experiments, in which, during the exponential growth phase, the inlet gas was changed from air to nitrogen. It was found that the mutant produced mannitol only under anaerobic conditions. However, anaerobic growth was not regained, which was probably due to the excessive accumulation of mannitol in the cells.},
  author       = {Costenoble, R and Adler, L and Niklasson, C and Lidén, Gunnar},
  issn         = {1567-1364},
  language     = {eng},
  number       = {1},
  pages        = {17--25},
  publisher    = {Elsevier},
  series       = {FEMS Yeast Research},
  title        = {Engineering of the metabolism of Saccharomyces cerevisiae for anaerobic production of mannitol,},
  url          = {http://dx.doi.org/},
  volume       = {3},
  year         = {2003},
}