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Laser-induced thermal stress and the heat shock response in neural cells

Emohare, Osa; Hafez, Moustafa I; Sandison, Ann; Coombs, Richard R H and McCarthy, Ian LU (2004) In Acta Orthopaedica Scandinavica 75(5). p.610-617
Abstract
Background The Ho: YAG laser is used extensively in orthopedic surgery. It offers a minimally invasive method of ablating tissue with precision. Previous studies have explored the effects of laser use on temperature during experimental foraminoplasty. To date, there has been limited work on the effects of thermal stress on cells in this context.



Material and methods Cells were exposed either to heated medium or the Ho: YAG laser in the high-power mode. Heated medium was used as a stressor by (I) exposing groups of cells to a constant temperature of 45°C for varying lengths of time: 5, 10, 15 and 20 min, and (II) exposing cells for a fixed length of time (5 min) to varying temperatures: 45°C, 55°C, 65°C with a control... (More)
Background The Ho: YAG laser is used extensively in orthopedic surgery. It offers a minimally invasive method of ablating tissue with precision. Previous studies have explored the effects of laser use on temperature during experimental foraminoplasty. To date, there has been limited work on the effects of thermal stress on cells in this context.



Material and methods Cells were exposed either to heated medium or the Ho: YAG laser in the high-power mode. Heated medium was used as a stressor by (I) exposing groups of cells to a constant temperature of 45°C for varying lengths of time: 5, 10, 15 and 20 min, and (II) exposing cells for a fixed length of time (5 min) to varying temperatures: 45°C, 55°C, 65°C with a control treated at 37°C. A third group was subjected to direct laser treatment. The effects of the treatments were assessed using trypan blue staining as a measure of viability and immunocytochemistry was used to measure changes in heat shock protein (HSP) expression.



Results There was a negative correlation between cell viability and HSP expression, and between cell viability and the severity of the treatment.



Interpretation Our findings suggest a possible role for the Ho: YAG laser in spinal foraminoplasty based on the high level of cell viability in the treatment regimen that most closely mirrored the clinical application of the laser. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Orthopaedica Scandinavica
volume
75
issue
5
pages
610 - 617
publisher
Taylor & Francis
external identifiers
  • wos:000224805200017
  • pmid:15513496
  • scopus:7044233507
ISSN
0001-6470
DOI
10.1080/00016470410001510
language
English
LU publication?
yes
id
6528b7b1-8127-4cf9-b051-a2d40b6069b1 (old id 129581)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=15513496&dopt=Abstract
date added to LUP
2007-07-03 11:08:08
date last changed
2017-01-01 08:03:52
@article{6528b7b1-8127-4cf9-b051-a2d40b6069b1,
  abstract     = {Background The Ho: YAG laser is used extensively in orthopedic surgery. It offers a minimally invasive method of ablating tissue with precision. Previous studies have explored the effects of laser use on temperature during experimental foraminoplasty. To date, there has been limited work on the effects of thermal stress on cells in this context.<br/><br>
<br/><br>
Material and methods Cells were exposed either to heated medium or the Ho: YAG laser in the high-power mode. Heated medium was used as a stressor by (I) exposing groups of cells to a constant temperature of 45°C for varying lengths of time: 5, 10, 15 and 20 min, and (II) exposing cells for a fixed length of time (5 min) to varying temperatures: 45°C, 55°C, 65°C with a control treated at 37°C. A third group was subjected to direct laser treatment. The effects of the treatments were assessed using trypan blue staining as a measure of viability and immunocytochemistry was used to measure changes in heat shock protein (HSP) expression.<br/><br>
<br/><br>
Results There was a negative correlation between cell viability and HSP expression, and between cell viability and the severity of the treatment.<br/><br>
<br/><br>
Interpretation Our findings suggest a possible role for the Ho: YAG laser in spinal foraminoplasty based on the high level of cell viability in the treatment regimen that most closely mirrored the clinical application of the laser.},
  author       = {Emohare, Osa and Hafez, Moustafa I and Sandison, Ann and Coombs, Richard R H and McCarthy, Ian},
  issn         = {0001-6470},
  language     = {eng},
  number       = {5},
  pages        = {610--617},
  publisher    = {Taylor & Francis},
  series       = {Acta Orthopaedica Scandinavica},
  title        = {Laser-induced thermal stress and the heat shock response in neural cells},
  url          = {http://dx.doi.org/10.1080/00016470410001510},
  volume       = {75},
  year         = {2004},
}