Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique
(1986) In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 34(11). p.1399-1403- Abstract
- An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1296161
- author
- Egesten, Arne LU ; Alumets, Jan LU ; von Mecklenburg, C ; Palmegren, M and Olsson, Inge LU
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- volume
- 34
- issue
- 11
- pages
- 1399 - 1403
- publisher
- Histochemical Society
- external identifiers
-
- scopus:0022466359
- ISSN
- 1551-5044
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Faculty of Medicine (000022000), Respiratory Medicine and Allergology (013230111), Division of Infection Medicine (BMC) (013024020), Division of Hematology and Transfusion Medicine (013041100), Pathology, (Lund) (013030000)
- id
- 2561d4ba-338d-4f8b-ace0-6872535659cc (old id 1296161)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/3772075
- http://jhc.sagepub.com/content/34/11/1399.long
- date added to LUP
- 2016-04-04 13:05:33
- date last changed
- 2024-01-13 05:56:06
@article{2561d4ba-338d-4f8b-ace0-6872535659cc, abstract = {{An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.}}, author = {{Egesten, Arne and Alumets, Jan and von Mecklenburg, C and Palmegren, M and Olsson, Inge}}, issn = {{1551-5044}}, language = {{eng}}, number = {{11}}, pages = {{1399--1403}}, publisher = {{Histochemical Society}}, series = {{The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society}}, title = {{Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique}}, url = {{http://www.ncbi.nlm.nih.gov/pubmed/3772075}}, volume = {{34}}, year = {{1986}}, }