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The bactericidal/permeability-increasing protein (BPI) is present in specific granules of human eosinophils

Calafat, J; Janssen, H; Tool, A; Dentener, M A; Knol, E F; Rosenberg, H F and Egesten, Arne LU (1998) In Blood 91(12). p.4770-4775
Abstract
Eosinophils participate in the inflammatory response seen in allergy and parasitic infestation, but a role in host defense against bacterial infection is not settled. The bactericidal/permeability-increasing protein (BPI) has been demonstrated in neutrophils and it exerts bacteriostatic and bactericidal effects against a wide variety of Gram-negative bacterial species. Using the Western blot technique, a 55-kD band, corresponding to BPI, was detected in lysates from both neutrophils and eosinophils. The localization of BPI in immature and mature eosinophils was investigated using immunoelectron microscopy. BPI was found in immature and mature specific granules of eosinophils and was detected in phagosomes as well, indicating release of the... (More)
Eosinophils participate in the inflammatory response seen in allergy and parasitic infestation, but a role in host defense against bacterial infection is not settled. The bactericidal/permeability-increasing protein (BPI) has been demonstrated in neutrophils and it exerts bacteriostatic and bactericidal effects against a wide variety of Gram-negative bacterial species. Using the Western blot technique, a 55-kD band, corresponding to BPI, was detected in lysates from both neutrophils and eosinophils. The localization of BPI in immature and mature eosinophils was investigated using immunoelectron microscopy. BPI was found in immature and mature specific granules of eosinophils and was detected in phagosomes as well, indicating release of the protein from the granules into the phagosomes. Using a specific enzyme-linked immunosorbent assay, eosinophils were shown to contain 179 ng of BPI/5 x 10(6) eosinophils compared with 710 ng BPI/5 x 10(6) neutrophils. The presence of BPI in eosinophils suggests a role for these cells in host defense against Gram-negative bacterial invasion or may suggest a role for BPI against parasitic infestation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
91
issue
12
pages
4770 - 4775
publisher
American Society of Hematology
external identifiers
  • scopus:0031840239
ISSN
1528-0020
language
English
LU publication?
yes
id
066055bb-dc54-41ab-a720-8d1b7cc8e97e (old id 1296519)
alternative location
http://bloodjournal.hematologylibrary.org/cgi/content/abstract/91/12/4770
date added to LUP
2009-07-31 11:25:02
date last changed
2017-06-11 03:50:11
@article{066055bb-dc54-41ab-a720-8d1b7cc8e97e,
  abstract     = {Eosinophils participate in the inflammatory response seen in allergy and parasitic infestation, but a role in host defense against bacterial infection is not settled. The bactericidal/permeability-increasing protein (BPI) has been demonstrated in neutrophils and it exerts bacteriostatic and bactericidal effects against a wide variety of Gram-negative bacterial species. Using the Western blot technique, a 55-kD band, corresponding to BPI, was detected in lysates from both neutrophils and eosinophils. The localization of BPI in immature and mature eosinophils was investigated using immunoelectron microscopy. BPI was found in immature and mature specific granules of eosinophils and was detected in phagosomes as well, indicating release of the protein from the granules into the phagosomes. Using a specific enzyme-linked immunosorbent assay, eosinophils were shown to contain 179 ng of BPI/5 x 10(6) eosinophils compared with 710 ng BPI/5 x 10(6) neutrophils. The presence of BPI in eosinophils suggests a role for these cells in host defense against Gram-negative bacterial invasion or may suggest a role for BPI against parasitic infestation.},
  author       = {Calafat, J and Janssen, H and Tool, A and Dentener, M A and Knol, E F and Rosenberg, H F and Egesten, Arne},
  issn         = {1528-0020},
  language     = {eng},
  number       = {12},
  pages        = {4770--4775},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {The bactericidal/permeability-increasing protein (BPI) is present in specific granules of human eosinophils},
  volume       = {91},
  year         = {1998},
}