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Minimal exclusion of plasma membrane proteins during retroviral envelope formation

Hammarstedt, Maria LU ; Wallengren, K; Pedersen, K W; Roos, N and Garoff, H (2000) In Proceedings of the National Academy of Sciences 97(13). p.7527-7532
Abstract
The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism of this phenomenon has remained elusive. We have purified Moloney murine leukemia virus Gag particles by sedimentation in an iodixanol gradient and donor PMs by flotation in a sucrose gradient and compared their protein compositions at equal lipid basis. We found that most PM proteins are present at similar density in both membranes. The... (More)
The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism of this phenomenon has remained elusive. We have purified Moloney murine leukemia virus Gag particles by sedimentation in an iodixanol gradient and donor PMs by flotation in a sucrose gradient and compared their protein compositions at equal lipid basis. We found that most PM proteins are present at similar density in both membranes. The inclusion of PM proteins was unaffected by incorporation of Env protein into the envelope of the Gag particles and whether these were produced at high or low level in the cells. These findings indicate that most PM proteins become incorporated into the retrovirus envelope without significant sorting. This feature of retrovirus assembly should be considered when studying retrovirus functions and developing retrovirus vectors. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Proceedings of the National Academy of Sciences
volume
97
issue
13
pages
7527 - 7532
publisher
National Acad Sciences
external identifiers
  • wos:000087811600107
  • scopus:0034691083
ISSN
1091-6490
DOI
10.1073/pnas.120051597
language
English
LU publication?
no
id
feb11e72-2a23-49d1-9f73-2212e69f4dc2 (old id 1297022)
date added to LUP
2009-07-15 13:33:57
date last changed
2017-01-01 04:43:10
@article{feb11e72-2a23-49d1-9f73-2212e69f4dc2,
  abstract     = {The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism of this phenomenon has remained elusive. We have purified Moloney murine leukemia virus Gag particles by sedimentation in an iodixanol gradient and donor PMs by flotation in a sucrose gradient and compared their protein compositions at equal lipid basis. We found that most PM proteins are present at similar density in both membranes. The inclusion of PM proteins was unaffected by incorporation of Env protein into the envelope of the Gag particles and whether these were produced at high or low level in the cells. These findings indicate that most PM proteins become incorporated into the retrovirus envelope without significant sorting. This feature of retrovirus assembly should be considered when studying retrovirus functions and developing retrovirus vectors.},
  author       = {Hammarstedt, Maria and Wallengren, K and Pedersen, K W and Roos, N and Garoff, H},
  issn         = {1091-6490},
  language     = {eng},
  number       = {13},
  pages        = {7527--7532},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Minimal exclusion of plasma membrane proteins during retroviral envelope formation},
  url          = {http://dx.doi.org/10.1073/pnas.120051597},
  volume       = {97},
  year         = {2000},
}