The effects of platelet apheresis in total hip replacement surgery on platelet activation
(2002) In Acta Anaesthesiologica Scandinavica 46(1). p.68-73- Abstract
- Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.
Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.
Platelet activation was evaluated as the population expressing... (More) - Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.
Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.
Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.
Results and conclusions: A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1–23% in peroperative activation. PRP harvest did not significantly alter platelet activity.
The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value ± SD 86% ± 7.5%). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1297529
- author
- Ekbäck, G ; Edlund, B ; Smolowics, A ; Axelsson, K ; Kjellberg, J and Schött, Ulf LU
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Anaesthesiologica Scandinavica
- volume
- 46
- issue
- 1
- pages
- 68 - 73
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000174421600012
- scopus:0036191237
- ISSN
- 0001-5172
- DOI
- 10.1046/j.0001-5172.2001.00367.x
- project
- Koagulation vid kirurgi och kritisk sjukdom
- language
- English
- LU publication?
- no
- id
- 56e80032-dfba-4056-aacf-b918e507836e (old id 1297529)
- date added to LUP
- 2016-04-01 12:38:45
- date last changed
- 2022-03-06 02:22:06
@article{56e80032-dfba-4056-aacf-b918e507836e,
abstract = {{Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.<br/><br>
<br/><br>
Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.<br/><br>
<br/><br>
Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.<br/><br>
<br/><br>
Results and conclusions: A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1–23% in peroperative activation. PRP harvest did not significantly alter platelet activity.<br/><br>
<br/><br>
The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value ± SD 86% ± 7.5%).}},
author = {{Ekbäck, G and Edlund, B and Smolowics, A and Axelsson, K and Kjellberg, J and Schött, Ulf}},
issn = {{0001-5172}},
language = {{eng}},
number = {{1}},
pages = {{68--73}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Anaesthesiologica Scandinavica}},
title = {{The effects of platelet apheresis in total hip replacement surgery on platelet activation}},
url = {{http://dx.doi.org/10.1046/j.0001-5172.2001.00367.x}},
doi = {{10.1046/j.0001-5172.2001.00367.x}},
volume = {{46}},
year = {{2002}},
}