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The effects of platelet apheresis in total hip replacement surgery on platelet activation

Ekbäck, G; Edlund, B; Smolowics, A; Axelsson, K; Kjellberg, J and Schött, Ulf LU (2002) In Acta Anaesthesiologica Scandinavica 46(1). p.68-73
Abstract
Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.



Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.



Platelet activation was evaluated as the population expressing... (More)
Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.



Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.



Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.



Results and conclusions: A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1–23% in peroperative activation. PRP harvest did not significantly alter platelet activity.



The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value ± SD 86% ± 7.5%). (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Anaesthesiologica Scandinavica
volume
46
issue
1
pages
68 - 73
publisher
Wiley-Blackwell
external identifiers
  • wos:000174421600012
  • scopus:0036191237
ISSN
0001-5172
DOI
10.1046/j.0001-5172.2001.00367.x
language
English
LU publication?
no
id
56e80032-dfba-4056-aacf-b918e507836e (old id 1297529)
date added to LUP
2009-07-14 12:02:38
date last changed
2017-01-01 05:19:45
@article{56e80032-dfba-4056-aacf-b918e507836e,
  abstract     = {Background: Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.<br/><br>
<br/><br>
Methods: Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group.<br/><br>
<br/><br>
Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.<br/><br>
<br/><br>
Results and conclusions: A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1–23% in peroperative activation. PRP harvest did not significantly alter platelet activity.<br/><br>
<br/><br>
The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value ± SD 86% ± 7.5%).},
  author       = {Ekbäck, G and Edlund, B and Smolowics, A and Axelsson, K and Kjellberg, J and Schött, Ulf},
  issn         = {0001-5172},
  language     = {eng},
  number       = {1},
  pages        = {68--73},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Anaesthesiologica Scandinavica},
  title        = {The effects of platelet apheresis in total hip replacement surgery on platelet activation},
  url          = {http://dx.doi.org/10.1046/j.0001-5172.2001.00367.x},
  volume       = {46},
  year         = {2002},
}