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Identification of Naf1/ABIN-1 among TNF-alpha-induced expressed genes in human synoviocytes using oligonucleotide microarrays

Gallagher, Joanne; Howlin, Jillian LU ; McCarthy, Conor; Murphy, Evelyn P; Bresnihan, Barry; FitzGerald, Oliver; Godson, Catherine; Brady, Hugh R and Martin, Finian (2003) In FEBS Letters 551(1-3). p.8-12
Abstract
The cytokine tumor necrosis factor alpha (TNF-alpha) is a critical effector of the pathogenesis of rheumatoid arthritis (RA). We used oligonucleotide microarray (OM) analysis to assess TNF-alpha-modulated gene expression in cultured primary human synoviocytes in vitro. Genes identified include cytokines and inflammatory mediators, extracellular matrix and adhesion molecules, cell cycle and proliferation related proteins, transcription related proteins, and apoptotic mediators. OM identified 1185 differentially expressed genes in TNF-alpha-treated synoviocytes. The regulation of Nef-associated factor-1 (Naf1), an A20-binding, nuclear factor kappa B (NFkappaB) inhibitory protein was probed further given its putative role as an endogenous... (More)
The cytokine tumor necrosis factor alpha (TNF-alpha) is a critical effector of the pathogenesis of rheumatoid arthritis (RA). We used oligonucleotide microarray (OM) analysis to assess TNF-alpha-modulated gene expression in cultured primary human synoviocytes in vitro. Genes identified include cytokines and inflammatory mediators, extracellular matrix and adhesion molecules, cell cycle and proliferation related proteins, transcription related proteins, and apoptotic mediators. OM identified 1185 differentially expressed genes in TNF-alpha-treated synoviocytes. The regulation of Nef-associated factor-1 (Naf1), an A20-binding, nuclear factor kappa B (NFkappaB) inhibitory protein was probed further given its putative role as an endogenous brake for the expression of some TNF-alpha-driven genes. Naf1 mRNA levels were higher in synovial biopsies from patients with active RA and seronegative arthropathy than in those from patients with osteoarthritis. These findings underscore the value of transcriptome analysis in cytokine-activated synoviocyte cultures in vitro as a means of identifying disease-associated genes in human arthritis, and implicate Naf1 as a potential modulator of TNF-alpha bioactivity in RA. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
551
issue
1-3
pages
8 - 12
publisher
Wiley-Blackwell
external identifiers
  • wos:000185308600003
  • scopus:0142039051
ISSN
1873-3468
DOI
language
English
LU publication?
no
id
611e0749-5bf0-4de5-b39b-fbf5cc0cf28b (old id 1297605)
date added to LUP
2009-07-14 09:07:44
date last changed
2018-05-29 11:07:07
@article{611e0749-5bf0-4de5-b39b-fbf5cc0cf28b,
  abstract     = {The cytokine tumor necrosis factor alpha (TNF-alpha) is a critical effector of the pathogenesis of rheumatoid arthritis (RA). We used oligonucleotide microarray (OM) analysis to assess TNF-alpha-modulated gene expression in cultured primary human synoviocytes in vitro. Genes identified include cytokines and inflammatory mediators, extracellular matrix and adhesion molecules, cell cycle and proliferation related proteins, transcription related proteins, and apoptotic mediators. OM identified 1185 differentially expressed genes in TNF-alpha-treated synoviocytes. The regulation of Nef-associated factor-1 (Naf1), an A20-binding, nuclear factor kappa B (NFkappaB) inhibitory protein was probed further given its putative role as an endogenous brake for the expression of some TNF-alpha-driven genes. Naf1 mRNA levels were higher in synovial biopsies from patients with active RA and seronegative arthropathy than in those from patients with osteoarthritis. These findings underscore the value of transcriptome analysis in cytokine-activated synoviocyte cultures in vitro as a means of identifying disease-associated genes in human arthritis, and implicate Naf1 as a potential modulator of TNF-alpha bioactivity in RA.},
  author       = {Gallagher, Joanne and Howlin, Jillian and McCarthy, Conor and Murphy, Evelyn P and Bresnihan, Barry and FitzGerald, Oliver and Godson, Catherine and Brady, Hugh R and Martin, Finian},
  issn         = {1873-3468},
  language     = {eng},
  number       = {1-3},
  pages        = {8--12},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Identification of Naf1/ABIN-1 among TNF-alpha-induced expressed genes in human synoviocytes using oligonucleotide microarrays},
  url          = {http://dx.doi.org/},
  volume       = {551},
  year         = {2003},
}