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Growth hormone enhances proinflammatory cytokine production by monocytes in whole blood

Uronen-Hansson, Heli LU ; Allen, M L; Lichtarowicz-Krynska, E; Aynsley-Green, A; Cole, T J; Hoiden-Guthenberg, I; Fryklund, L and Klein, N (2003) In Growth Hormone & Igf Research 13(5). p.282-286
Abstract
Growth hormone (GH) has been used as anabolic therapy to treat catabolic patients. In a recent study, however, administration of high doses of GH to critically ill adults was associated with an increase in morbidity and mortality. Preponderance of septic shock and uncontrolled infections as causes of death in these patients suggests an immuno-modulatory effect of GH. Our hypothesis was that GH treatment may modulate the production of proinflammatory cytokines, which are implicated in sepsis. In our study, human monocytes in whole blood were activated with lipopolysaccaharide (LPS) (1-100 ng/ml) purified from a clinical isolate of group B Neisseria meningitidis in the presence of a high dose of GH (100 ng/ml). The subsequent proinflammatory... (More)
Growth hormone (GH) has been used as anabolic therapy to treat catabolic patients. In a recent study, however, administration of high doses of GH to critically ill adults was associated with an increase in morbidity and mortality. Preponderance of septic shock and uncontrolled infections as causes of death in these patients suggests an immuno-modulatory effect of GH. Our hypothesis was that GH treatment may modulate the production of proinflammatory cytokines, which are implicated in sepsis. In our study, human monocytes in whole blood were activated with lipopolysaccaharide (LPS) (1-100 ng/ml) purified from a clinical isolate of group B Neisseria meningitidis in the presence of a high dose of GH (100 ng/ml). The subsequent proinflammatory cytokine response was analysed by intracellular cytokine staining and flow cytometry. Our results show that GH enhances IL1-alpha, IL-6 and TNF-alpha production by LPS activated monocytes in whole blood. The modulation of cytokines by GH may be responsible for the adverse consequences of GH in critically ill patients. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Growth Hormone & Igf Research
volume
13
issue
5
pages
282 - 286
publisher
Elsevier
external identifiers
  • wos:000185548800008
  • scopus:0141854288
ISSN
1532-2238
DOI
10.1016/S1096-6374(03)00034-0
language
English
LU publication?
no
id
4f774034-ed1b-4898-9a6a-19f92af8e1fe (old id 1297668)
date added to LUP
2009-07-14 10:06:01
date last changed
2018-02-04 03:28:26
@article{4f774034-ed1b-4898-9a6a-19f92af8e1fe,
  abstract     = {Growth hormone (GH) has been used as anabolic therapy to treat catabolic patients. In a recent study, however, administration of high doses of GH to critically ill adults was associated with an increase in morbidity and mortality. Preponderance of septic shock and uncontrolled infections as causes of death in these patients suggests an immuno-modulatory effect of GH. Our hypothesis was that GH treatment may modulate the production of proinflammatory cytokines, which are implicated in sepsis. In our study, human monocytes in whole blood were activated with lipopolysaccaharide (LPS) (1-100 ng/ml) purified from a clinical isolate of group B Neisseria meningitidis in the presence of a high dose of GH (100 ng/ml). The subsequent proinflammatory cytokine response was analysed by intracellular cytokine staining and flow cytometry. Our results show that GH enhances IL1-alpha, IL-6 and TNF-alpha production by LPS activated monocytes in whole blood. The modulation of cytokines by GH may be responsible for the adverse consequences of GH in critically ill patients.},
  author       = {Uronen-Hansson, Heli and Allen, M L and Lichtarowicz-Krynska, E and Aynsley-Green, A and Cole, T J and Hoiden-Guthenberg, I and Fryklund, L and Klein, N},
  issn         = {1532-2238},
  language     = {eng},
  number       = {5},
  pages        = {282--286},
  publisher    = {Elsevier},
  series       = {Growth Hormone & Igf Research},
  title        = {Growth hormone enhances proinflammatory cytokine production by monocytes in whole blood},
  url          = {http://dx.doi.org/10.1016/S1096-6374(03)00034-0},
  volume       = {13},
  year         = {2003},
}