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Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina

Blixt, Frank W. LU ; Radziwon-Balicka, Aneta; Edvinsson, Lars LU and Warfvinge, Karin LU (2017) In Experimental Eye Research 161. p.124-131
Abstract

Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer.... (More)

Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CGRP, CLR, Flow cytometry, Immunohistochemistry, RAMP1, Retina
in
Experimental Eye Research
volume
161
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85021154432
  • wos:000408072800015
ISSN
0014-4835
DOI
10.1016/j.exer.2017.06.002
language
English
LU publication?
yes
id
12c6a46c-9696-4009-b9a4-1a2def2be300
date added to LUP
2017-07-26 12:35:47
date last changed
2017-09-18 11:39:46
@article{12c6a46c-9696-4009-b9a4-1a2def2be300,
  abstract     = {<p>Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.</p>},
  author       = {Blixt, Frank W. and Radziwon-Balicka, Aneta and Edvinsson, Lars and Warfvinge, Karin},
  issn         = {0014-4835},
  keyword      = {CGRP,CLR,Flow cytometry,Immunohistochemistry,RAMP1,Retina},
  language     = {eng},
  month        = {08},
  pages        = {124--131},
  publisher    = {Elsevier},
  series       = {Experimental Eye Research},
  title        = {Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina},
  url          = {http://dx.doi.org/10.1016/j.exer.2017.06.002},
  volume       = {161},
  year         = {2017},
}