Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina
Blixt, Frank W. LU ; Radziwon-Balicka, Aneta ; Edvinsson, Lars LU and Warfvinge, Karin LU
(2017)
In Experimental Eye Research
161.
p.124-131
- Abstract
Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer.... (More)
Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.
(Less)
- author
- Blixt, Frank W.
LU
; Radziwon-Balicka, Aneta
; Edvinsson, Lars
LU
and Warfvinge, Karin
LU
- organization
- publishing date
- 2017-08-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CGRP, CLR, Flow cytometry, Immunohistochemistry, RAMP1, Retina
- in
- Experimental Eye Research
- volume
- 161
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85021154432
- pmid:28603014
- wos:000408072800015
- ISSN
- 0014-4835
- DOI
- 10.1016/j.exer.2017.06.002
- language
- English
- LU publication?
- yes
- id
- 12c6a46c-9696-4009-b9a4-1a2def2be300
- date added to LUP
- 2017-07-26 12:35:47
- date last changed
- 2024-05-26 19:41:18
@article{12c6a46c-9696-4009-b9a4-1a2def2be300,
abstract = {{<p>Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Müller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.</p>}},
author = {{Blixt, Frank W. and Radziwon-Balicka, Aneta and Edvinsson, Lars and Warfvinge, Karin}},
issn = {{0014-4835}},
keywords = {{CGRP; CLR; Flow cytometry; Immunohistochemistry; RAMP1; Retina}},
language = {{eng}},
month = {{08}},
pages = {{124--131}},
publisher = {{Elsevier}},
series = {{Experimental Eye Research}},
title = {{Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina}},
url = {{http://dx.doi.org/10.1016/j.exer.2017.06.002}},
doi = {{10.1016/j.exer.2017.06.002}},
volume = {{161}},
year = {{2017}},
}