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Characterization of the cell surface glycolipid from Spirochaeta aurantia.

Paul, Catherine LU ; Lyle, Elizabeth; Beveridge, Terry; Tapping, Richard; Kropinski, Andrew and Vinogradov, Evgeny (2009) In Glycoconjugate Journal 26. p.1097-1108
Abstract
Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D:... (More)
Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D: -manno-heptose (DDHep), and a novel branched tetradeoxydecose monosaccharide, which we proposed to call aurantose (Aur). The carbohydrate structure of LGLA was extremely complex and consisted of the repeating units built of 11 monosaccharides, arrangement of nine of them was determined as: [Formula: see text] which wasdeduced from the NMR and chemical data on the LGLA and its fragments, obtained by various degradations. Tentative position of two remaining sugars is proposed. LGLA was negative for gelation of Limulus amebocyte lysate, did not contain lipid A, and was unable to activate any known Toll-like receptors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Glycoconjugate Journal
volume
26
pages
1097 - 1108
publisher
Springer
external identifiers
  • wos:000272784000001
  • pmid:19214746
  • scopus:77649243019
ISSN
1573-4986
DOI
10.1007/s10719-009-9230-4
language
English
LU publication?
yes
id
e9d93559-2a61-4335-9a94-25e73890e67a (old id 1302586)
date added to LUP
2009-03-27 13:48:33
date last changed
2017-01-01 06:11:43
@article{e9d93559-2a61-4335-9a94-25e73890e67a,
  abstract     = {Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D: -manno-heptose (DDHep), and a novel branched tetradeoxydecose monosaccharide, which we proposed to call aurantose (Aur). The carbohydrate structure of LGLA was extremely complex and consisted of the repeating units built of 11 monosaccharides, arrangement of nine of them was determined as: [Formula: see text] which wasdeduced from the NMR and chemical data on the LGLA and its fragments, obtained by various degradations. Tentative position of two remaining sugars is proposed. LGLA was negative for gelation of Limulus amebocyte lysate, did not contain lipid A, and was unable to activate any known Toll-like receptors.},
  author       = {Paul, Catherine and Lyle, Elizabeth and Beveridge, Terry and Tapping, Richard and Kropinski, Andrew and Vinogradov, Evgeny},
  issn         = {1573-4986},
  language     = {eng},
  pages        = {1097--1108},
  publisher    = {Springer},
  series       = {Glycoconjugate Journal},
  title        = {Characterization of the cell surface glycolipid from Spirochaeta aurantia.},
  url          = {http://dx.doi.org/10.1007/s10719-009-9230-4},
  volume       = {26},
  year         = {2009},
}