Characterization of the cell surface glycolipid from Spirochaeta aurantia.
(2009) In Glycoconjugate Journal 26. p.1097-1108- Abstract
- Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D:... (More)
- Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D: -manno-heptose (DDHep), and a novel branched tetradeoxydecose monosaccharide, which we proposed to call aurantose (Aur). The carbohydrate structure of LGLA was extremely complex and consisted of the repeating units built of 11 monosaccharides, arrangement of nine of them was determined as: [Formula: see text] which wasdeduced from the NMR and chemical data on the LGLA and its fragments, obtained by various degradations. Tentative position of two remaining sugars is proposed. LGLA was negative for gelation of Limulus amebocyte lysate, did not contain lipid A, and was unable to activate any known Toll-like receptors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1302586
- author
- Paul, Catherine
LU
; Lyle, Elizabeth ; Beveridge, Terry ; Tapping, Richard ; Kropinski, Andrew and Vinogradov, Evgeny
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Glycoconjugate Journal
- volume
- 26
- pages
- 1097 - 1108
- publisher
- Springer
- external identifiers
-
- wos:000272784000001
- pmid:19214746
- scopus:77649243019
- pmid:19214746
- ISSN
- 1573-4986
- DOI
- 10.1007/s10719-009-9230-4
- language
- English
- LU publication?
- yes
- id
- e9d93559-2a61-4335-9a94-25e73890e67a (old id 1302586)
- date added to LUP
- 2016-04-01 14:21:09
- date last changed
- 2024-01-10 02:37:24
@article{e9d93559-2a61-4335-9a94-25e73890e67a, abstract = {{Spirochaeta aurantia is a free-living saprophytic spirochete that grows easily in simple laboratory media, and thus can be used as a model for the investigation of surface carbohydrate structures in spirochetae, which are normally not available in sufficient amounts. Freeze-substitution electron microscopy indicated the presence of a capsule-like material projecting from the surface of S. aurantia. Extraction of cells gave two major glycolipids, the one with a higher molecular mass glycolipid was designated large glycolipid A (LGLA). LGLA contained small amount of branched and unsaturated O-linked fatty acids, L: -rhamnose, L: -fucose, D: -xylose, D: -mannose, D: -glucosamine, D: -glycero-D: -gluco-heptose (DDglcHep), D: -glycero-D: -manno-heptose (DDHep), and a novel branched tetradeoxydecose monosaccharide, which we proposed to call aurantose (Aur). The carbohydrate structure of LGLA was extremely complex and consisted of the repeating units built of 11 monosaccharides, arrangement of nine of them was determined as: [Formula: see text] which wasdeduced from the NMR and chemical data on the LGLA and its fragments, obtained by various degradations. Tentative position of two remaining sugars is proposed. LGLA was negative for gelation of Limulus amebocyte lysate, did not contain lipid A, and was unable to activate any known Toll-like receptors.}}, author = {{Paul, Catherine and Lyle, Elizabeth and Beveridge, Terry and Tapping, Richard and Kropinski, Andrew and Vinogradov, Evgeny}}, issn = {{1573-4986}}, language = {{eng}}, pages = {{1097--1108}}, publisher = {{Springer}}, series = {{Glycoconjugate Journal}}, title = {{Characterization of the cell surface glycolipid from Spirochaeta aurantia.}}, url = {{http://dx.doi.org/10.1007/s10719-009-9230-4}}, doi = {{10.1007/s10719-009-9230-4}}, volume = {{26}}, year = {{2009}}, }