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Antibody-based microarrays.

Wingren, Christer LU and Borrebaeck, Carl LU (2009) In Methods in Molecular Biology 509. p.57-84
Abstract
Antibody-based microarrays are a new powerful proteomic technology that can be used to generate rapid and detailed expression profiles of defined sets of protein analytes in complex samples as well as high-resolution portraits of entire proteomes. Miniaturized micro- and nanoarrays can be printed with numerous antibodies carrying the desired specificities. Multiplexed and ultra-sensitive assays, specifically targeting several analytes in a single experiment, can be performed, while consuming only minute amounts of the sample. The array images generated can then be converted into protein expression profiles, or maps, revealing the detailed composition of the sample. This promising proteomic research tool will thus provide unique... (More)
Antibody-based microarrays are a new powerful proteomic technology that can be used to generate rapid and detailed expression profiles of defined sets of protein analytes in complex samples as well as high-resolution portraits of entire proteomes. Miniaturized micro- and nanoarrays can be printed with numerous antibodies carrying the desired specificities. Multiplexed and ultra-sensitive assays, specifically targeting several analytes in a single experiment, can be performed, while consuming only minute amounts of the sample. The array images generated can then be converted into protein expression profiles, or maps, revealing the detailed composition of the sample. This promising proteomic research tool will thus provide unique opportunities for e.g. disease proteomics, biomarker discovery, disease diagnostics, and patient stratification. This review describes the antibody-based microarray technology and applications thereof. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
509
pages
57 - 84
publisher
Springer
external identifiers
  • pmid:19212715
  • scopus:62449083668
ISSN
1940-6029
DOI
10.1007/978-1-59745-372-1_5
project
CREATE Health
language
English
LU publication?
yes
id
5e6dec6f-d03b-4930-8663-10a72217b3e8 (old id 1302641)
date added to LUP
2009-03-05 10:53:33
date last changed
2017-08-13 03:36:56
@article{5e6dec6f-d03b-4930-8663-10a72217b3e8,
  abstract     = {Antibody-based microarrays are a new powerful proteomic technology that can be used to generate rapid and detailed expression profiles of defined sets of protein analytes in complex samples as well as high-resolution portraits of entire proteomes. Miniaturized micro- and nanoarrays can be printed with numerous antibodies carrying the desired specificities. Multiplexed and ultra-sensitive assays, specifically targeting several analytes in a single experiment, can be performed, while consuming only minute amounts of the sample. The array images generated can then be converted into protein expression profiles, or maps, revealing the detailed composition of the sample. This promising proteomic research tool will thus provide unique opportunities for e.g. disease proteomics, biomarker discovery, disease diagnostics, and patient stratification. This review describes the antibody-based microarray technology and applications thereof.},
  author       = {Wingren, Christer and Borrebaeck, Carl},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {57--84},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Antibody-based microarrays.},
  url          = {http://dx.doi.org/10.1007/978-1-59745-372-1_5},
  volume       = {509},
  year         = {2009},
}