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Flow-injection assay of the pathogenic bacteria using lectin-based quartz crystal microbalance biosensor

Safina, Gulnara LU ; van Lier, Margret and Danielsson, Bengt LU (2008) 14h International Conference on Flow Injection Analysis and Related Techniques In Talanta 77(2). p.468-472
Abstract
A novel flow-injection assay of the pathogenic enterobacteria using novel lectin-based quartz crystal microbalance (QCM) biosensor has been proposed. The biosensing part of the analytical device contained the lectins - Concanavalin A, lectins from Ulex europeus, Maackia amurensis, Lens culinaris, wheat germ agglutinin - immobilized on the gold surface of quartz crystal electrode which served as a transducer. The immobilization of lectins was carried out using amine Coupling Oil the surface of the crystal modified with 11-mercaproundecanoic acid. The biosensor makes it possible to identify the presence of different bacterial using the lectins immobilized on the surface of QCM crystal which bind specifically to the certain oligosaccharides... (More)
A novel flow-injection assay of the pathogenic enterobacteria using novel lectin-based quartz crystal microbalance (QCM) biosensor has been proposed. The biosensing part of the analytical device contained the lectins - Concanavalin A, lectins from Ulex europeus, Maackia amurensis, Lens culinaris, wheat germ agglutinin - immobilized on the gold surface of quartz crystal electrode which served as a transducer. The immobilization of lectins was carried out using amine Coupling Oil the surface of the crystal modified with 11-mercaproundecanoic acid. The biosensor makes it possible to identify the presence of different bacterial using the lectins immobilized on the surface of QCM crystal which bind specifically to the certain oligosaccharides present on the cell wall of the bacteria injected. The working conditions of the biosensor - pH of buffer Solutions, concentration of the immobilized lectins, dilution of the bacterial cells, regeneration solution and flow rate - were optimized. The use of solution of glycine (pH 2.5) makes it possible to remove the formed complex from the crystal Surface to make it reusable and ready for the next experiment. The proposed biosensor is able to detect 10(3) cells. The flow-injection assay of the bacterial cells takes about 30 min. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Flow-injection analysis, Immobilized, lectins, Campylobacter jejuni, Helicobacter pylori, Pathogenic bacteria, Quartz crystal microbalance
in
Talanta
volume
77
issue
2
pages
468 - 472
publisher
Elsevier
conference name
14h International Conference on Flow Injection Analysis and Related Techniques
external identifiers
  • wos:000261416500003
  • scopus:54349091495
ISSN
0039-9140
DOI
10.1016/j.talanta.2008.03.033
language
English
LU publication?
yes
id
95ab9a37-05c5-44e9-ab70-2a0999d70657 (old id 1304361)
date added to LUP
2009-03-23 14:46:47
date last changed
2017-07-23 04:24:56
@inproceedings{95ab9a37-05c5-44e9-ab70-2a0999d70657,
  abstract     = {A novel flow-injection assay of the pathogenic enterobacteria using novel lectin-based quartz crystal microbalance (QCM) biosensor has been proposed. The biosensing part of the analytical device contained the lectins - Concanavalin A, lectins from Ulex europeus, Maackia amurensis, Lens culinaris, wheat germ agglutinin - immobilized on the gold surface of quartz crystal electrode which served as a transducer. The immobilization of lectins was carried out using amine Coupling Oil the surface of the crystal modified with 11-mercaproundecanoic acid. The biosensor makes it possible to identify the presence of different bacterial using the lectins immobilized on the surface of QCM crystal which bind specifically to the certain oligosaccharides present on the cell wall of the bacteria injected. The working conditions of the biosensor - pH of buffer Solutions, concentration of the immobilized lectins, dilution of the bacterial cells, regeneration solution and flow rate - were optimized. The use of solution of glycine (pH 2.5) makes it possible to remove the formed complex from the crystal Surface to make it reusable and ready for the next experiment. The proposed biosensor is able to detect 10(3) cells. The flow-injection assay of the bacterial cells takes about 30 min. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.},
  author       = {Safina, Gulnara and van Lier, Margret and Danielsson, Bengt},
  booktitle    = {Talanta},
  issn         = {0039-9140},
  keyword      = {Flow-injection analysis,Immobilized,lectins,Campylobacter jejuni,Helicobacter pylori,Pathogenic bacteria,Quartz crystal microbalance},
  language     = {eng},
  number       = {2},
  pages        = {468--472},
  publisher    = {Elsevier},
  title        = {Flow-injection assay of the pathogenic bacteria using lectin-based quartz crystal microbalance biosensor},
  url          = {http://dx.doi.org/10.1016/j.talanta.2008.03.033},
  volume       = {77},
  year         = {2008},
}