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Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA

Salman, Salah LU ; Srimathi, Soundararajan LU ; Safina, Gulnara LU ; Satoh, Ikuo and Danielsson, Bengt LU (2008) 14h International Conference on Flow Injection Analysis and Related Techniques In Talanta 77(2). p.490-493
Abstract
The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were... (More)
The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were analyzed. The novelty of the described work is the rapid set up of glucose analysis using hydroxyapatite as a reusable immobilization Support in a now injection thermal biosensor without any need for covalent immobilization or chemical cross-linking. The property of hydroxyapatite to adsorb and desorb proteins as a function of the buffer pH and ionic Strength makes in situ enzyme reloading or exchange possible. The standard curves were obtained within few hours with a high degree of reproducibility and the enzyme remained fully active even after 3 months. (C) 2008 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Enzyme thermistor, Flow-injection analysis, Glucose, Immobilization, biosensor, Hydroxapatite
in
Talanta
volume
77
issue
2
pages
490 - 493
publisher
Elsevier
conference name
14h International Conference on Flow Injection Analysis and Related Techniques
external identifiers
  • wos:000261416500007
  • scopus:54349096845
ISSN
0039-9140
DOI
10.1016/j.talanta.2008.04.003
language
English
LU publication?
yes
id
c5ca7f82-045d-42c9-9fbe-83e138a28ae2 (old id 1304366)
date added to LUP
2009-03-23 14:49:13
date last changed
2017-10-01 04:10:28
@inproceedings{c5ca7f82-045d-42c9-9fbe-83e138a28ae2,
  abstract     = {The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were analyzed. The novelty of the described work is the rapid set up of glucose analysis using hydroxyapatite as a reusable immobilization Support in a now injection thermal biosensor without any need for covalent immobilization or chemical cross-linking. The property of hydroxyapatite to adsorb and desorb proteins as a function of the buffer pH and ionic Strength makes in situ enzyme reloading or exchange possible. The standard curves were obtained within few hours with a high degree of reproducibility and the enzyme remained fully active even after 3 months. (C) 2008 Elsevier B.V. All rights reserved.},
  author       = {Salman, Salah and Srimathi, Soundararajan and Safina, Gulnara and Satoh, Ikuo and Danielsson, Bengt},
  booktitle    = {Talanta},
  issn         = {0039-9140},
  keyword      = {Enzyme thermistor,Flow-injection analysis,Glucose,Immobilization,biosensor,Hydroxapatite},
  language     = {eng},
  number       = {2},
  pages        = {490--493},
  publisher    = {Elsevier},
  title        = {Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA},
  url          = {http://dx.doi.org/10.1016/j.talanta.2008.04.003},
  volume       = {77},
  year         = {2008},
}