Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA
(2008) 14h International Conference on Flow Injection Analysis and Related Techniques 77(2). p.490-493- Abstract
- The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were... (More)
- The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were analyzed. The novelty of the described work is the rapid set up of glucose analysis using hydroxyapatite as a reusable immobilization Support in a now injection thermal biosensor without any need for covalent immobilization or chemical cross-linking. The property of hydroxyapatite to adsorb and desorb proteins as a function of the buffer pH and ionic Strength makes in situ enzyme reloading or exchange possible. The standard curves were obtained within few hours with a high degree of reproducibility and the enzyme remained fully active even after 3 months. (C) 2008 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1304366
- author
- Salman, Salah LU ; Srimathi, Soundararajan LU ; Safina, Gulnara LU ; Satoh, Ikuo and Danielsson, Bengt LU
- organization
- publishing date
- 2008
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Enzyme thermistor, Flow-injection analysis, Glucose, Immobilization, biosensor, Hydroxapatite
- host publication
- Talanta
- volume
- 77
- issue
- 2
- pages
- 490 - 493
- publisher
- Elsevier
- conference name
- 14h International Conference on Flow Injection Analysis and Related Techniques
- conference location
- Berlin, Germany
- conference dates
- 2007-09-02 - 2007-09-07
- external identifiers
-
- wos:000261416500007
- scopus:54349096845
- ISSN
- 0039-9140
- DOI
- 10.1016/j.talanta.2008.04.003
- language
- English
- LU publication?
- yes
- id
- c5ca7f82-045d-42c9-9fbe-83e138a28ae2 (old id 1304366)
- date added to LUP
- 2016-04-01 13:37:35
- date last changed
- 2022-03-21 19:36:04
@inproceedings{c5ca7f82-045d-42c9-9fbe-83e138a28ae2,
abstract = {{The application of the easily available and inexpensive chromatographic matrix hydroxyapatite for reusable and reversible immobilization of enzymes for enzyme thermistor-based flow injection analysis of glucose and Urea was tested. The immobilization was achieved by simple affinity adsorption of glucose oxidase and urease by a suitable pH-induced alteration of the protein charge. A linear detection range of 0.05-8.0 mM was observed for glucose estimation depending oil the sensitivity and sample loop parameters with a detection limit of 0.05 mM. A broad detection range of 0.5-50 mM was observed for urea using the flow injection calorimetric biosensor. Some real samples like commercial soft drink, syrups, honey and serum samples were analyzed. The novelty of the described work is the rapid set up of glucose analysis using hydroxyapatite as a reusable immobilization Support in a now injection thermal biosensor without any need for covalent immobilization or chemical cross-linking. The property of hydroxyapatite to adsorb and desorb proteins as a function of the buffer pH and ionic Strength makes in situ enzyme reloading or exchange possible. The standard curves were obtained within few hours with a high degree of reproducibility and the enzyme remained fully active even after 3 months. (C) 2008 Elsevier B.V. All rights reserved.}},
author = {{Salman, Salah and Srimathi, Soundararajan and Safina, Gulnara and Satoh, Ikuo and Danielsson, Bengt}},
booktitle = {{Talanta}},
issn = {{0039-9140}},
keywords = {{Enzyme thermistor; Flow-injection analysis; Glucose; Immobilization; biosensor; Hydroxapatite}},
language = {{eng}},
number = {{2}},
pages = {{490--493}},
publisher = {{Elsevier}},
title = {{Hydroxyapatite as a novel reversible in situ adsorption matrix for enzyme thermistor-based FIA}},
url = {{http://dx.doi.org/10.1016/j.talanta.2008.04.003}},
doi = {{10.1016/j.talanta.2008.04.003}},
volume = {{77}},
year = {{2008}},
}