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The Human Protein Atlas - a tool for pathology

Ponten, F.; Jirström, Karin LU and Uhlen, M. (2008) In Journal of Pathology 216(4). p.387-393
Abstract
Tissue-based diagnostics and research is incessantly evolving with the development of new molecular tools. It has long been realized that immunohistochemistry can add an important new level of information on top of morphology and that protein expression patterns in a cancer may yield crucial diagnostic and prognostic information. We have generated an immunohistochemistry-based map of protein expression profiles in normal tissues, cancer and cell lines. For each antibody, altogether 708 spots of tissues and cells are analysed and the resulting images and data are presented as freely available in the Human Protein Atlas (www.proteinatias.org). The new version 4 of the atlas, including more than 5 million images of immunohistochemically... (More)
Tissue-based diagnostics and research is incessantly evolving with the development of new molecular tools. It has long been realized that immunohistochemistry can add an important new level of information on top of morphology and that protein expression patterns in a cancer may yield crucial diagnostic and prognostic information. We have generated an immunohistochemistry-based map of protein expression profiles in normal tissues, cancer and cell lines. For each antibody, altogether 708 spots of tissues and cells are analysed and the resulting images and data are presented as freely available in the Human Protein Atlas (www.proteinatias.org). The new version 4 of the atlas, including more than 5 million images of immunohistochemically stained tissues and cells, is based on 6122 antibodies, representing 5011 human proteins encoded by approximately 25% of the human genome. The genecentric database includes a putative classification of proteins in various protein classes, both functional classes, such as kinases or transcription factors and project-related classes, such as candidate genes for cancer or cardiovascular diseases. For each of the internally generated antibodies, the exact antigen sequence is presented, together with a visualization of application-specific validation data, including a protein array assay, western blot analysis, immunohistochemistry and, in most cases, immunofluorescent-based confocal microscopy. The updated version also includes new search algorithms to allow complex queries regarding expression profiles, protein classes and chromosome location. Thus, the presented Human Protein Atlas provides a resource for pathology-based biomedical research, including protein science and biomarker discovery. Copyright (C) 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
translational research, protein, atlas, biomarker, antibody, proteome, immunohistochemistry
in
Journal of Pathology
volume
216
issue
4
pages
387 - 393
publisher
John Wiley & Sons
external identifiers
  • wos:000261509200001
  • scopus:57149110826
ISSN
0022-3417
DOI
10.1002/path.2440
language
English
LU publication?
yes
id
c16fffbc-c7eb-4e1b-a87d-842135bb3af1 (old id 1305523)
date added to LUP
2009-03-23 12:56:40
date last changed
2017-11-12 03:23:38
@misc{c16fffbc-c7eb-4e1b-a87d-842135bb3af1,
  abstract     = {Tissue-based diagnostics and research is incessantly evolving with the development of new molecular tools. It has long been realized that immunohistochemistry can add an important new level of information on top of morphology and that protein expression patterns in a cancer may yield crucial diagnostic and prognostic information. We have generated an immunohistochemistry-based map of protein expression profiles in normal tissues, cancer and cell lines. For each antibody, altogether 708 spots of tissues and cells are analysed and the resulting images and data are presented as freely available in the Human Protein Atlas (www.proteinatias.org). The new version 4 of the atlas, including more than 5 million images of immunohistochemically stained tissues and cells, is based on 6122 antibodies, representing 5011 human proteins encoded by approximately 25% of the human genome. The genecentric database includes a putative classification of proteins in various protein classes, both functional classes, such as kinases or transcription factors and project-related classes, such as candidate genes for cancer or cardiovascular diseases. For each of the internally generated antibodies, the exact antigen sequence is presented, together with a visualization of application-specific validation data, including a protein array assay, western blot analysis, immunohistochemistry and, in most cases, immunofluorescent-based confocal microscopy. The updated version also includes new search algorithms to allow complex queries regarding expression profiles, protein classes and chromosome location. Thus, the presented Human Protein Atlas provides a resource for pathology-based biomedical research, including protein science and biomarker discovery. Copyright (C) 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.},
  author       = {Ponten, F. and Jirström, Karin and Uhlen, M.},
  issn         = {0022-3417},
  keyword      = {translational research,protein,atlas,biomarker,antibody,proteome,immunohistochemistry},
  language     = {eng},
  number       = {4},
  pages        = {387--393},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Pathology},
  title        = {The Human Protein Atlas - a tool for pathology},
  url          = {http://dx.doi.org/10.1002/path.2440},
  volume       = {216},
  year         = {2008},
}